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| Sample: | Mianserin 56 ng/ml |
| Inj. vol.: | 500 µl serum |
| Extraction column : | BioTrap 500 MS, 20x4.0 mm |
| Extraction mobile phase: | 4% 2-propanol in 10mM ammonium acetate buffer, pH 10 (adjusted with ammoniumhydroxide) |
| Flow: | 3.2 ml/min |
| Analytical column: | Hypersil Elite C18, 150x4.6 mm, 5 µm + Elite guard, 10x4 mm, 5 µm |
| Analytical mobile phase: | 40% acetonitrile in 50 mM formic acid |
| Flow: | 1.0 ml/min |
| Detection: | Post column derivatization: Beam Boost UV= 254 nm 20 m x 0.3 mm I.D. Fluorescense: ex= 270 nm, em= 430 nm |
Analysis Program
| Switching valve position | Analysis step | Time |
| 1. Extraction position | sample extraction | 2.5 min |
| 2. Elution position | transfer of analyte | 4 min |
| 3. Extraction position | separation and reequilibration | 7.5 min |
If you have any questions regarding the above, don´t hesitate to contact Chrom Tech!
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