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| Sample: | Dextromethorphan (55 ng/ml) in serum |
| Inj. vol.: | 200 µl |
| Extraction column : | BioTrap 500 C8, 20x4.0 mm |
| Extraction mobile phase: | 4% 2-propanol and 5 mM sod. octanesulfonic acid in 20 m M sod. ph. b. pH 7.0 |
| Flow: | 1.6 ml/min |
| Analytical column: | Zorbax XDB-C8, 150 x 4.6 mm, 5 µm + guard |
| Analytical mobile phase: | 30% acetonitrile and 2 mM sod. octanesulfonic acid in 116 mM sod.ph.b. pH 2.8 |
| Flow: | 1.0 ml/min |
| Detection: | Fluorescence, ex 220 nm, 305 nm |
Analysis Program
| Switching valve position | Analysis step | Time |
| 1. Extraction position | sample extraction | 3 min |
| 2. Elution position | transfer of analyte | 5 min |
| 3. Extraction position | separation and reequilibration | 7 min |
If you have any questions regarding the above, don´t hesitate to contact Chrom Tech!
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