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CHIRAL-CBH
A chiral HPLC-column for direct resolution of enantiomers
Cellobiohydrolase (CBH) is a stable enzyme, which has been immobilized onto 5 micrometer spherical silica particles. This is a reversed-phase column, used for the direct separation of enantiomers. The column is preferantially used for the separation of enantiomers of basic drugs from many compound classes. CHIRAL-CBH separates preferentially compounds containing one or more basic nitrogens together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester etc.).
Download Now Chiral CBH Presentation 2.7 MB
A reference list with literature references is presented.
In the Application index application examples are presented. The chromatograms are demonstrated together with information about the column used and the mobile phase conditions.
Application Areas
At the drug companies CHIRAL-CBH is used for the analysis of
enantiomeric purity and for bioanalysis. An interesting and fast
growing area is isolation of pure enantiomers on semipreparative
columns.
Mobile Phases
The mobile phases are mixtures of phosphate or acetate buffers
and organic solvents as 2-propanol or acetonitrile. The retention
and the enantioselectivity can be regulated by changes in pH,
buffer concentration and organic modifier (nature and
concentration).
Dimensions
CHIRAL-CBH columns are available with inner diameters 2, 3, 4,
and 10 mm in lengths of 100 and 150 mm. Guards with length of 10
mm and the inner diameters 2 and 3 mm are also available. For
more details see Ordering
Information.
If you have any questions regarding the above, donīt hesitate to contact Chrom Tech!
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