Interactive High Performance Liquid
Chromatography
This recently upgraded and expanded
training package, written by an experienced practical
chromatographer, contains a wealth of information about
all aspects of HPLC.
It gives comprehensive descriptions, with animated,
interactive demonstrations, of the principles,
instrumentation and practical applications of High
Performance Liquid Chromatography, with self-test
questions on each module.
Its
five modules are as follows:
1. Chromatographic Theory
Introduction: column
chromatography, chromatograms.
Separation theory: retention time,
capacity factor, selectivity factor, resolution.
Column efficiency: peak shapes, band
spreading, column efficiency, efficiency &
resolution.
Column dispersion mechanisms: band
broadening, Van Deemter equation, eddy diffusion,
longitudinal diffusion, mass transfer effects, reduced
terms.
2. HPLC
Instrumentation
Introduction:
instrumentation overview.
Eluent delivery system: initial
conditions, syringe pump, single piston pump, twin piston
pumps, ( series & parallel), gradient pumping, (high
& low pressure mixing).
Sample introduction: sample injection
valve, injection loop.
Columns: guard & analytical.
Detectors: UV-vis, diode array,
fluorescence, refractive index, electrochemical,
conductivity, amperometric, coulometric.
3. Analysis using
HPLC
Sample preparation:
sample clean up, solid phase extraction, derivatization.
Qualitative analysis: peak
identification, sample identification.
Quantitative analysis: peak integration,
area normalization, external standards, internal
standards, example of internal standards.
Method development: initial
considerations, choice of method, column, detector &
eluent, gradient elution, developing a gradient
separation. Interactive examples of method development,
with organic and ionic samples.
4. Separation
Modes & Columns
Classification of methods:
silica as a stationary phase, chemical modification of
silica.
Adsorption chromatography: silica as an
adsorbent, adsorption chromatography, applications.
Reversed-phase HPLC: bonded stationary
phases, reversed-phase eluents, ion-suppression,
applications.
Ion-pair chromatography: ion pair
reagents.
Ion exchange chromatography: ion
exchange properties of silica, ion exchange mechanism,
ionic strength, effect of eluent pH, variables
controlling chromatography retention.
Size exclusion: calibration curves,
applications.
5. Questions &
Answers
Ten
questions on each of:
HPLC Instrumentation
HPLC Separation Nodes & Analysis
HPLC Theory
Interactive HPLC is one of a range of
training packages for analytical chemistry: also
available are Interactive GC, GC Applications &
Method Development, ICP, AAS & MS. They are suitable
for both university students and laboratory staff, giving
sufficient emphasis to both theory and practice. Network
versions are available.
There is now a wealth of research
demonstrating the advantages of computer-based training
as compared to traditional methods: increased
understanding and retention, shorter training time,
constant availability, flexibility of use and
considerable cost savings. Training is no longer
dependent upon having access to instruments, and can be
tailored to individual requirements.
Trainees can watch the animation
demonstrating the working of the instrument; adjust
parameters to see their effect; pursue their own lines of
inquiry; and return to areas of uncertainty. The software
has a free flow, open-ended structure which enables the
user to browse freely through its pages, or to follow the
programmed order. Thus a useful session may last 10
minutes or several hours.
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