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Extraction procedure for ketoconazole in serum with RePeat 25 mg SPE Cartridge
Conditioning:
1-2 ml 1% triethylamine in ethyl acetate
1 ml distilled water
Sample application:
1 ml sample (serum mixed 1:1 with 4% 2-propanol in 30 mM
sodium hydroxide)
Washing:
2 x 1 ml 4% 2-propanol in 30 mM sodium hydroxide
0.5 ml distilled water
Elution:
1 ml 1% triethylamine in ethyl acetate.
Evaporate and reconstitute.
A chromatogram of ketoconazole extracted from serum is given below
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