AdvanceBio Glycan Mapping
Agilent AdvanceBio Glycan Mapping
The Agilent AdvanceBio Glycan Mapping column family is engineered specifically for fast, high-resolution profiling of released glycans in biopharmaceutical development and quality control. Built on advanced particle technologies, these columns deliver exceptional efficiency, sharp peak shape, and highly reproducible migration patterns—allowing analysts to confidently monitor glycosylation changes that directly impact drug safety, efficacy, and stability.
- Ultra-fast analysis — Generate detailed glycan maps in under 10 minutes to accelerate characterization and lot-release workflows.
- High-resolution structural insight — Precisely resolve both labeled and unlabeled glycans to detect subtle shifts caused by culture conditions, purification steps, or formulation changes.
- Flexible particle technologies — 2.7 µm superficially porous particles (SPP) provide high resolution with lower backpressure, ensuring compatibility with virtually all LC systems. 1.8 µm fully porous particles (FPP) deliver maximum resolving power for UHPLC workflows requiring the highest separation efficiency.
- Lot-to-lot reproducibility — Each column is QA-tested using a 2-AB–labeled IgG N-linked glycan sample to ensure consistent retention, selectivity, and glycan pattern fidelity.
Optimized for biopharma, biosimilars, vaccine development, and glycoprotein characterization, the AdvanceBio Glycan Mapping portfolio enables confident, quantitative profiling across development and QC environments.
For help selecting the ideal particle format or method setup, contact Chrom Tech’s technical support team.
RP-mAb (Reversed-Phase Monoclonal Antibody Chromatography)
A reversed-phase mode optimized for intact monoclonal antibodies and large biomolecules. Uses wide-pore particles to improve resolution of heavy/light chains, fragments, and high-mass species.
450Å Wide-Pore Particles
Extra-wide pore particles designed to accommodate large biomolecules, improving mass transfer, peak shape, and resolution for proteins, mAbs, and glycoproteins.
Superficially Porous Particles (SPP)
Also known as core-shell particles, SPP offer UHPLC-level efficiency with lower backpressure. Ideal for high-resolution biomolecule separations on standard LC systems.
Embedded Polar Group (EPG)
A bonding technology incorporating a polar functional group within the alkyl chain to enhance selectivity, improve wetting in aqueous conditions, and deliver cleaner peak shapes for hydrophilic analytes.
Diphenyl Stationary Phase
An aromatic phase offering π–π interactions and alternative selectivity for mAbs, peptides, and hydrophobic proteins—useful for confirming identity or resolving closely related variants.
What makes AdvanceBio Glycan Mapping columns ideal for glycan analysis?
AdvanceBio Glycan Mapping columns deliver fast, high-resolution separation of labeled and unlabeled glycans, enabling clear detection of glycosylation changes with excellent reproducibility and lot-to-lot consistency.
Should I choose the 2.7 µm SPP or 1.8 µm fully porous particle format?
The 2.7 µm superficially porous particles provide high resolution with lower backpressure and are compatible with all LC systems. The 1.8 µm fully porous particles deliver maximum resolving power for UHPLC workflows requiring the tightest peak shapes and fastest run times.
What types of samples are typically analyzed with these columns?
These columns are optimized for released N-linked glycans from biotherapeutics such as mAbs, biosimilars, fusion proteins, and other glycoprotein-based biologics. They support both 2-AB and other fluorescently labeled glycan workflows.
How does Agilent ensure reproducibility between batches?
Each lot is QA-tested using a 2-AB labeled IgG N-linked glycan standard. This ensures consistent peak shape, retention behavior, and resolution across lots—critical for regulated QC and comparability studies.
Product List
| SKU | Name | LC Column Phase | Particle Size | Inner Diameter (ID) | Length |
|---|---|---|---|---|---|
SKU
858700-913
|
Name
858700-913 - AdvanceBio Glycan Map 2.1x100, 1.8um
|
LC Column Phase
Amide HILIC
|
Particle Size
1.8 um
|
Inner Diameter (ID)
2.1 mm
|
Length
100 mm
|
SKU
859700-913
|
Name
859700-913 - AdvanceBio Glycan Map 2.1x150, 1.8um
|
LC Column Phase
Amide HILIC
|
Particle Size
1.8 um
|
Inner Diameter (ID)
2.1 mm
|
Length
150 mm
|
SKU
821725-906
|
Name
821725-906 - AdvanceBio Glycan Map 2.1mm, 2.7um fast guard
|
LC Column Phase
Amide HILIC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
2.1 mm
|
Length
5 mm
|
SKU
821725-905
|
Name
821725-905 - AdvanceBio Glycan Map 2.1mm, 1.8um fast guard
|
LC Column Phase
Amide HILIC
|
Particle Size
1.8 um
|
Inner Diameter (ID)
2.1 mm
|
Length
5 mm
|
SKU
683775-913
|
Name
683775-913 - AdvanceBio Glycan Map 2.1x150, 2.7um
|
LC Column Phase
Amide HILIC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
2.1 mm
|
Length
150 mm
|
SKU
680975-913
|
Name
680975-913 - AdvanceBio Glycan Map 4.6x250, 2.7um
|
LC Column Phase
Amide HILIC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
4.6 mm
|
Length
250 mm
|
SKU
685975-913
|
Name
685975-913 - AdvanceBio Glycan Map 4.6x100, 2.7um
|
LC Column Phase
Amide HILIC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
4.6 mm
|
Length
100 mm
|
SKU
685775-913
|
Name
685775-913 - AdvanceBio Glycan Map 2.1x100, 2.7um
|
LC Column Phase
Amide HILIC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
2.1 mm
|
Length
100 mm
|
SKU
683975-913
|
Name
683975-913 - AdvanceBio Glycan Map4.6x150, 2.7um
|
LC Column Phase
Amide HILIC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
4.6 mm
|
Length
150 mm
|
SKU
651750-913
|
Name
651750-913 - AdvanceBio Glycan Map 2.1x250, 2.7um
|
LC Column Phase
Amide HILIC
|
Particle Size
2.7 um
|
Inner Diameter (ID)
2.1 mm
|
Length
250 mm
|