AdvanceBio RP-mAb

Agilent AdvanceBio RP-mAb Columns

Agilent AdvanceBio RP-mAb columns are purpose-engineered for high-resolution reversed-phase analysis of monoclonal antibodies and large biomolecules. Built on a 3.5 µm superficially porous particle with an enlarged 450 Å pore size, these columns deliver the ideal balance of speed, sensitivity, and robustness for intact mAb, subunit, and fragment characterization.

The wide-pore SPP architecture enhances mass transfer for large proteins, enabling sharper peaks, reduced carryover, and improved quantitation—even for complex mAb therapeutics. Multiple bonded-phase chemistries, including C4, SB-C8, and Diphenyl, provide flexible selectivity for intact, reduced, and stressed-sample analyses.

  • Improved accuracy — 3.5 µm SPP with 450 Å pore increases resolution for intact mAbs and large fragments.
  • Selectable chemistries — C4, SB-C8, and Diphenyl phases optimize retention and selectivity for various mAb workflows.
  • Longer column lifetime — 2 µm inlet frit and rugged packed bed reduce fouling and extend usability.
  • Low carryover — Wide-pore design minimizes adsorption of large proteins and glycoforms.

From intact antibody mapping to Fc/Fab and light/heavy chain separations, AdvanceBio RP-mAb columns provide the reproducible resolution required for biopharmaceutical development, QC, and characterization. Ideal for LC/MS workflows and high-throughput analytical laboratories.

For help selecting the best AdvanceBio column for your mAb workflow, contact Chrom Tech.

Key Definitions ▸

Superficially Porous Particles (SPP)

SPP, also called core–shell particles, feature a solid core with a thin porous shell. They provide UHPLC-like resolution with lower backpressure—ideal for large biomolecules such as monoclonal antibodies (mAbs) and fragments.

Wide Pore (450 Å)

A large pore size designed for intact proteins and mAbs. Wider pores reduce restricted diffusion and improve resolution of heavy and light chain fragments.

C4, SB-C8 & Diphenyl Selectivity

Optimized bonded phases for large biomolecules: C4 for intact mAbs, SB-C8 for fragments and hydrophobic variants, Diphenyl for aromatic interactions and subtle structural differences.

2 μm Inlet Frit

A tighter inlet frit prevents clogging from aggregates or sample particulates, extending column lifetime and maintaining sharp peak shape during protein analysis.

Intact mAb & Fragment Analysis

RP-mAb columns are optimized for intact antibodies, heavy/light chain fragments, Fc/Fab components, and other large biomolecules requiring high recovery, low carry-over, and reliable reproducibility.

Frequently Asked Questions ▸

What makes the AdvanceBio RP-mAb column ideal for monoclonal antibodies?

The 3.5 µm superficially porous particles with a wide 450 Å pore size provide high-resolution separations of intact mAbs and fragments. This design improves peak shape, reduces carry-over, and enhances the accuracy of glycoform and variant profiling.

Which phases are available in the RP-mAb series?

AdvanceBio RP-mAb columns are available in C4, SB-C8, and Diphenyl phases, offering tailored selectivity for intact mAbs, light and heavy chains, Fc/Fab fragments, and other large biomolecules.

How does the frit design improve column lifetime?

A durable 2 µm inlet frit resists clogging when analyzing protein-rich or complex matrices, protecting the packed bed and extending the usable life of the column—reducing replacement cost and downtime.

What applications benefit most from the RP-mAb column?

These columns excel in intact mAb analysis, reduced mAb characterization (light/heavy chains), biosimilar comparison, glycoform tracking, and QC workflows requiring fast, high-resolution separations of large biomolecules.

Product List

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SKU Name LC Column Phase Particle Size Inner Diameter (ID) Length
SKU
799975-944
Name
799975-944 - AdvanceBio RP-mAb Diphenyl 4.6 x 50 mm, 3.5 u
LC Column Phase
Diphenyl
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
50 mm
SKU
799975-904
Name
799975-904 - AdvanceBio RP-mAb C4 4.6 x 50 mm, 3.5 um
LC Column Phase
C4
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
50 mm
SKU
799775-944
Name
799775-944 - AdvanceBio RP-mAb Diphenyl 2.1 x 50 mm, 3.5um
LC Column Phase
Diphenyl
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
799775-904
Name
799775-904 - AdvanceBio RP-mAb C4 2.1 x 50 mm, 3.5 um
LC Column Phase
C4
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
793975-944
Name
793975-944 - AdvanceBio RP-mAb Diphenyl 4.6 x 150 mm, 3.5
LC Column Phase
Diphenyl
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
150 mm
SKU
793975-904
Name
793975-904 - AdvanceBio RP-mAb C4 4.6 x 150 mm, 3.5 um
LC Column Phase
C4
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
150 mm
SKU
797775-944
Name
797775-944 - AdvanceBio RP-mAb Diphenyl 2.1 x 75 mm, 3.5 u
LC Column Phase
Diphenyl
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
75 mm
SKU
797775-904
Name
797775-904 - AdvanceBio RP-mAb C4 2.1 x 75 mm, 3.5 um
LC Column Phase
C4
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
75 mm
SKU
793775-944
Name
793775-944 - AdvanceBio RP-mAb Diphenyl 2.1 x 150 mm, 3.5
LC Column Phase
Diphenyl
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
150 mm
SKU
793775-904
Name
793775-904 - AdvanceBio RP-mAb C4 2.1 x 150 mm, 3.5 um
LC Column Phase
C4
Particle Size
3.5 um
Inner Diameter (ID)
2.1 mm
Length
150 mm
SKU
795975-944
Name
795975-944 - AdvanceBio RP-mAb Diphenyl 4.6 x 100 mm, 3.5
LC Column Phase
Diphenyl
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
100 mm
SKU
795975-904
Name
795975-904 - AdvanceBio RP-mAb C4 4.6 x 100 mm, 3.5 um
LC Column Phase
C4
Particle Size
3.5 um
Inner Diameter (ID)
4.6 mm
Length
100 mm