AdvanceBio SEC

Agilent AdvanceBio SEC HPLC Bio Columns

Agilent AdvanceBio SEC (Size Exclusion Chromatography) columns are engineered to deliver highly accurate, reproducible quantitation of aggregates and fragments in peptides, monoclonal antibodies (mAbs), ADCs, and next-generation biotherapeutics. Designed to eliminate re-analysis and improve lab productivity, these columns offer unmatched consistency—column to column, batch to batch, and lab to lab—ensuring seamless method transfer across teams and sites.

Built on a unique hydrophilic, non-interacting surface technology, AdvanceBio SEC columns minimize secondary interactions—even with “sticky” biomolecules—providing clean, reliable molecular-size separations. This makes them an ideal choice for quality control, formulation studies, biosimilar development, and characterization of large, fragile biological entities.

AdvanceBio SEC 2.7 µm Columns

These robust columns are optimized for routine aggregate and fragment analysis of proteins and peptides. Originally offered in 300 Å and 130 Å pore sizes, they deliver sensitive, reliable results with minimal interaction between sample and stationary phase. New 500 Å and 1000 Å options extend the platform to support AAVs, VLPs, and other large biomolecular assemblies, enabling high-resolution characterization of emerging biotherapeutic modalities.

AdvanceBio SEC 1.9 µm Columns

For laboratories requiring maximum resolution and speed, the 1.9 µm series provides UHPLC-level efficiency. Stability up to 620 bar enables faster flow rates and higher throughput, while the 200 Å pore size is specifically designed for detailed mAb profiling—resolving monomer, dimer, and low-level fragments with exceptional clarity.

Whether you are characterizing traditional biologics or next-generation gene therapy products, Agilent AdvanceBio SEC columns deliver the reliability, robustness, and resolution needed for confident, regulatory-ready biomolecular analysis.

Agilent AdvanceBio SEC — Phase Description ▸
AdvanceBio SEC
Size Exclusion Chromatography • Hydrophilic, Diol-Bonded Silica • 300 Å & 130 Å Options • Aqueous Mobile Phases
▸

Agilent AdvanceBio SEC columns are engineered for high-resolution size-based separation of proteins, monoclonal antibodies (mAbs), aggregates, and biotherapeutics under native or near-native conditions. Unlike reversed-phase columns, SEC separates molecules strictly by hydrodynamic size, minimizing secondary interactions.

When to Choose AdvanceBio SEC
  • You need accurate quantitation of protein aggregates and fragments
  • You are performing mAb purity or stability studies
  • You require non-denaturing conditions for intact biomolecules
  • You need reproducible sizing with minimal adsorption
How It Works

Molecules are separated by their ability to penetrate porous particles. Larger molecules are excluded from the pores and elute first, while smaller species access more pore volume and elute later. The hydrophilic diol surface reduces unwanted protein–surface interactions, improving recovery and peak symmetry.

Key Specifications
Separation Mode Size Exclusion Chromatography (SEC)
Surface Chemistry Hydrophilic, diol-bonded silica
Pore Sizes Available Typically 130 Å (peptides/small proteins) and 300 Å (mAbs/large proteins)
Mobile Phase Aqueous buffers (e.g., phosphate or saline-based)
Primary Applications Aggregate analysis, fragment analysis, biosimilar comparison, protein purity testing
Interaction Mechanism Size-based exclusion (minimal secondary interactions)

Practical note: For optimal accuracy in aggregate quantitation, maintain appropriate ionic strength in the mobile phase to suppress unintended ionic interactions.

Product List

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SKU Name LC Column Phase Pore Size Inner Diameter (ID) Length
SKU
PL1180-5302
Name
PL1180-5302 - AdvanceBio SEC 1000 A, 2.7 um, 7.8 x 300
LC Column Phase
SEC
Pore Size
1000 A
Inner Diameter (ID)
7.8 mm
Length
300 mm
SKU
PL1180-5325
Name
PL1180-5325 - AdvanceBio SEC 500 A, 2.7 um, 7.8 x 300
LC Column Phase
SEC
Pore Size
500 A
Inner Diameter (ID)
7.8 mm
Length
300 mm
SKU
PL1180-1302
Name
PL1180-1302 - AdvanceBioSEC 1000 A, 2.7 um, 7.8x50mm, guard
LC Column Phase
SEC
Pore Size
1000 A
Inner Diameter (ID)
7.8 mm
Length
50 mm
SKU
PL1180-1325
Name
PL1180-1325 - AdvanceBio SEC 500 A, 2.7 um, 7.8x50mm, guard
LC Column Phase
SEC
Pore Size
500 A
Inner Diameter (ID)
7.8 mm
Length
50 mm
SKU
PL1580-5302
Name
PL1580-5302 - AdvanceBio SEC 1000 A, 2.7 um, 4.6x300mm
LC Column Phase
SEC
Pore Size
1000 A
Inner Diameter (ID)
4.6 mm
Length
300 mm
SKU
PL1580-5325
Name
PL1580-5325 - AdvanceBio SEC 500 A, 2.7 um, 4.6x300mm
LC Column Phase
SEC
Pore Size
500 A
Inner Diameter (ID)
4.6 mm
Length
300 mm
SKU
PL1580-3302
Name
PL1580-3302 - AdvanceBio SEC 1000 A, 2.7 um, 4.6x150mm
LC Column Phase
SEC
Pore Size
1000 A
Inner Diameter (ID)
4.6 mm
Length
150 mm
SKU
PL1580-3325
Name
PL1580-3325 - AdvanceBio SEC 500 A, 2.7 um, 4.6x150mm
LC Column Phase
SEC
Pore Size
500 A
Inner Diameter (ID)
4.6 mm
Length
150 mm
SKU
PL1580-1302
Name
PL1580-1302 - AdvanceBioSEC 1000 A, 2.7 um, 4.6x50mm, guard
LC Column Phase
SEC
Pore Size
1000 A
Inner Diameter (ID)
4.6 mm
Length
50 mm
SKU
PL1580-1325
Name
PL1580-1325 - AdvanceBio SEC 500 A, 2.7 um, 4.6x50mm, guard
LC Column Phase
SEC
Pore Size
500 A
Inner Diameter (ID)
4.6 mm
Length
50 mm
SKU
PL1580-5350
Name
PL1580-5350 - Zorbax AdvanceBio SEC 130A 2.7um 4.6x300mm
LC Column Phase
SEC
Pore Size
130 A
Inner Diameter (ID)
4.6 mm
Length
300 mm
SKU
PL1580-5301
Name
PL1580-5301 - Zorbax AdvanceBio SEC 300A 2.7um 4.6x300mm
LC Column Phase
SEC
Pore Size
300 A
Inner Diameter (ID)
4.6 mm
Length
300 mm
Key Definitions ▸
Size Exclusion Chromatography (SEC)
A chromatographic technique that separates molecules based on hydrodynamic size. Larger molecules elute first because they are excluded from pore volume, while smaller molecules penetrate the pores and elute later.
Diol-Bonded Silica
A hydrophilic stationary phase surface treatment that minimizes secondary hydrophobic or ionic interactions, improving protein recovery and maintaining native structure during SEC analysis.
300 Å vs 130 Å Pore Size
300 Å pores are optimized for large biomolecules such as monoclonal antibodies and aggregates. 130 Å pores are better suited for smaller proteins and peptides, offering improved resolution for lower molecular weight species.
Protein Aggregates
High-molecular-weight species formed by protein association. SEC is widely used to quantify aggregates in biopharmaceutical products as part of stability, comparability, and regulatory testing.
Native Conditions
Analytical conditions that preserve a protein’s natural conformation. SEC is commonly performed under aqueous buffer systems to maintain structural integrity during separation.
Frequently Asked Questions ▸
What is the primary purpose of AdvanceBio SEC columns?
AdvanceBio SEC columns are designed for size-based separation of proteins, monoclonal antibodies (mAbs), aggregates, and fragments. They are commonly used for aggregate quantitation, purity assessment, and stability studies in biopharmaceutical workflows.
How does SEC differ from reversed-phase chromatography?
SEC separates molecules strictly by hydrodynamic size, while reversed-phase chromatography separates by hydrophobic interactions. SEC minimizes secondary interactions, allowing proteins to be analyzed under near-native, non-denaturing conditions.
When should I choose 300 Å versus 130 Å pore size?
Use 300 Å for large biomolecules such as intact mAbs and aggregates. Choose 130 Å for smaller proteins and peptides where improved resolution of lower molecular weight species is required.
What mobile phases are recommended for AdvanceBio SEC?
AdvanceBio SEC columns are typically used with aqueous buffer systems such as phosphate or saline-based buffers. Proper ionic strength is important to suppress unintended ionic interactions and ensure accurate size-based separation.
Is AdvanceBio SEC compatible with LC/MS?
Yes. AdvanceBio SEC can be coupled with LC/MS systems for aggregate and fragment analysis, though volatile buffer systems may be required depending on the mass spectrometer and application requirements.