AdvanceBio SEC
Agilent AdvanceBio SEC HPLC Bio Columns
Agilent AdvanceBio SEC (Size Exclusion Chromatography) columns are engineered to deliver highly accurate, reproducible quantitation of aggregates and fragments in peptides, monoclonal antibodies (mAbs), ADCs, and next-generation biotherapeutics. Designed to eliminate re-analysis and improve lab productivity, these columns offer unmatched consistency—column to column, batch to batch, and lab to lab—ensuring seamless method transfer across teams and sites.
Built on a unique hydrophilic, non-interacting surface technology, AdvanceBio SEC columns minimize secondary interactions—even with “sticky” biomolecules—providing clean, reliable molecular-size separations. This makes them an ideal choice for quality control, formulation studies, biosimilar development, and characterization of large, fragile biological entities.
AdvanceBio SEC 2.7 µm Columns
These robust columns are optimized for routine aggregate and fragment analysis of proteins and peptides. Originally offered in 300 Å and 130 Å pore sizes, they deliver sensitive, reliable results with minimal interaction between sample and stationary phase. New 500 Å and 1000 Å options extend the platform to support AAVs, VLPs, and other large biomolecular assemblies, enabling high-resolution characterization of emerging biotherapeutic modalities.
AdvanceBio SEC 1.9 µm Columns
For laboratories requiring maximum resolution and speed, the 1.9 µm series provides UHPLC-level efficiency. Stability up to 620 bar enables faster flow rates and higher throughput, while the 200 Å pore size is specifically designed for detailed mAb profiling—resolving monomer, dimer, and low-level fragments with exceptional clarity.
Whether you are characterizing traditional biologics or next-generation gene therapy products, Agilent AdvanceBio SEC columns deliver the reliability, robustness, and resolution needed for confident, regulatory-ready biomolecular analysis.
Size Exclusion Chromatography (SEC)
A separation technique based on molecular size. Larger molecules elute first because they cannot enter the pores of the stationary phase, while smaller species take longer pathways and elute later.
Aggregate Analysis
The quantitation of higher-order species such as protein dimers, trimers, and larger aggregates—critical for biotherapeutic quality, stability, and regulatory compliance.
AAV / VLP Characterization
Analytical workflows used to assess size, aggregation, purity, and capsid integrity of adeno-associated viruses (AAV) and virus-like particles (VLPs), commonly used in gene therapy development.
Hydrophilic Surface Coating
A specialized surface treatment applied to some SEC phases to minimize secondary interactions and prevent adsorption of “sticky” biomolecules, ensuring accurate molecular-weight-based separation.
Pore Size (Å)
Indicates the diameter of pores in the stationary phase. Larger pore sizes (300–1000 Å) are required for large biomolecules such as mAbs, ADCs, AAVs, and protein aggregates.
What makes AdvanceBio SEC columns ideal for aggregate and fragment analysis?
AdvanceBio SEC columns use highly controlled particle technology and hydrophilic coatings to minimize secondary interactions, ensuring accurate, reproducible quantitation of aggregates, fragments, and monomers— even for complex modalities like mAbs, ADCs, and next-generation biotherapeutics.
How do I choose between 2.7 µm and 1.9 µm AdvanceBio SEC particles?
The 2.7 µm columns deliver robust resolution with low backpressure for routine SEC analyses, while the 1.9 µm columns offer ultra-high efficiency and faster run times—ideal for high-throughput labs and LC/MS workflows requiring maximum resolving power.
Which pore size should I use for my biomolecule?
Agilent offers SEC pore sizes from 130 Å to 1000 Å: 130 Å / 200 Å for peptides and mAb fragments, 300 Å for intact mAbs, 500 Å and 1000 Å for AAVs, VLPs, and large viral particles. Select the pore size that best matches the hydrodynamic radius of your analyte.
Are AdvanceBio SEC columns compatible across labs and instruments?
Yes. These columns are engineered for exceptional batch-to-batch reproducibility and platform compatibility, ensuring consistent results across instruments, departments, and global R&D or QC sites.
Do AdvanceBio SEC columns reduce the need for sample re-analysis?
Absolutely. Their robust design and minimized secondary interactions deliver stable retention and reliable quantitation—helping eliminate re-runs caused by column variability or surface interaction artifacts.
Product List
| SKU | Name | LC Column Phase | Pore Size | Inner Diameter (ID) | Length |
|---|---|---|---|---|---|
SKU
PL1180-5302
|
Name
PL1180-5302 - AdvanceBio SEC 1000 A, 2.7 um, 7.8 x 300
|
LC Column Phase
SEC
|
Pore Size
1000 A
|
Inner Diameter (ID)
7.8 mm
|
Length
300 mm
|
SKU
PL1180-5325
|
Name
PL1180-5325 - AdvanceBio SEC 500 A, 2.7 um, 7.8 x 300
|
LC Column Phase
SEC
|
Pore Size
500 A
|
Inner Diameter (ID)
7.8 mm
|
Length
300 mm
|
SKU
PL1180-1302
|
Name
PL1180-1302 - AdvanceBioSEC 1000 A, 2.7 um, 7.8x50mm, guard
|
LC Column Phase
SEC
|
Pore Size
1000 A
|
Inner Diameter (ID)
7.8 mm
|
Length
50 mm
|
SKU
PL1180-1325
|
Name
PL1180-1325 - AdvanceBio SEC 500 A, 2.7 um, 7.8x50mm, guard
|
LC Column Phase
SEC
|
Pore Size
500 A
|
Inner Diameter (ID)
7.8 mm
|
Length
50 mm
|
SKU
PL1580-5302
|
Name
PL1580-5302 - AdvanceBio SEC 1000 A, 2.7 um, 4.6x300mm
|
LC Column Phase
SEC
|
Pore Size
1000 A
|
Inner Diameter (ID)
4.6 mm
|
Length
300 mm
|
SKU
PL1580-5325
|
Name
PL1580-5325 - AdvanceBio SEC 500 A, 2.7 um, 4.6x300mm
|
LC Column Phase
SEC
|
Pore Size
500 A
|
Inner Diameter (ID)
4.6 mm
|
Length
300 mm
|
SKU
PL1580-3302
|
Name
PL1580-3302 - AdvanceBio SEC 1000 A, 2.7 um, 4.6x150mm
|
LC Column Phase
SEC
|
Pore Size
1000 A
|
Inner Diameter (ID)
4.6 mm
|
Length
150 mm
|
SKU
PL1580-3325
|
Name
PL1580-3325 - AdvanceBio SEC 500 A, 2.7 um, 4.6x150mm
|
LC Column Phase
SEC
|
Pore Size
500 A
|
Inner Diameter (ID)
4.6 mm
|
Length
150 mm
|
SKU
PL1580-1302
|
Name
PL1580-1302 - AdvanceBioSEC 1000 A, 2.7 um, 4.6x50mm, guard
|
LC Column Phase
SEC
|
Pore Size
1000 A
|
Inner Diameter (ID)
4.6 mm
|
Length
50 mm
|
SKU
PL1580-1325
|
Name
PL1580-1325 - AdvanceBio SEC 500 A, 2.7 um, 4.6x50mm, guard
|
LC Column Phase
SEC
|
Pore Size
500 A
|
Inner Diameter (ID)
4.6 mm
|
Length
50 mm
|
SKU
PL1580-5350
|
Name
PL1580-5350 - Zorbax AdvanceBio SEC 130A 2.7um 4.6x300mm
|
LC Column Phase
SEC
|
Pore Size
130 A
|
Inner Diameter (ID)
4.6 mm
|
Length
300 mm
|
SKU
PL1580-5301
|
Name
PL1580-5301 - Zorbax AdvanceBio SEC 300A 2.7um 4.6x300mm
|
LC Column Phase
SEC
|
Pore Size
300 A
|
Inner Diameter (ID)
4.6 mm
|
Length
300 mm
|