In anion exchange chromatography, the stationary bed has an ionically positive (+) charged surface while the sample ions are of negative (-) charge. This technique is used almost exclusively with ionic or ionizable samples. The stronger the negative charge on the sample, the stronger it will be attracted to the positive charge on the stationary phase, and thus the longer it will take to elute. Elution in ion chromatography is effected by mobile phase pH and ionic-strength, and, to a lesser extent, operation temperature. The ability to use the full pH range and elevated temperatures are distinct advantages compared to silica-based supports.
Hamilton offers six polymeric packing materials for anion exchange separations.
Hamilton PRP-X100 and PRP-X110 are highly stable, inert materials. The PRP-X100 can be used with virtually any HPLC or ion chromatograph, including dedicated IC units. PRP-X100 and PRP-X110 columns are well suited for use in systems employing suppressed/non-suppressed conductivity, electrochemical, UV, and ICP-MS detection.
PRP-X500 is a superficially porous polymeric anion exchange column designed for the separation, purification and isolation of proteins, peptides and DNA/RNA. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses typically seen on other commercially available protein HPLC columns.
PRP-X600 is a superficially porous weak-base anion exchange support that separates DNA oligomers according to negative charge. Because the PRP-X600 is a weak anion exchange (WAX) resin, the exchange capacity of the resin is pH dependent. Lowering the pH will reduce the binding of proteins, reducing the run time for a complete separation.
The Hamilton RCX-10 and RCX-30 carbohydrate analysis columns are designed for the isocratic or gradient separation of carbohydrates. The exchange capacity of the RCX-10/RCX-30 is greater than that of the PRP-X100, leading to characteristics better suited for the separation of carbohydrates.
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