Hamilton PRP-X100 and PRP-X110
Hamilton HxSil C18 columns are well end-capped for retention time reproducibility and durability. HxSil C18 columns exhibit greater retention than most columns and enhance the separation of compounds that are not sufficiently retained on other C18 columns. Hamilton HxSil C8 columns are well end-capped for retention time reproducibility and durability. The C8 demonstrates excellent selectivity needed for complex sample mixtures and gives shorter retention times than a C18.
Hamilton PRP-X500 and PRP-X600
PRP-X500 is a superficially porous polymeric anion exchange column designed for the separation, purification and isolation of proteins, peptides and DNA/RNA. The methacrylate polymeric coating of the PRP-X500 provides a more hydrophilic surface, preventing hydrophobic interaction sample losses typically seen on other commercially available protein HPLC columns.
PRP-X600 is a superficially porous weak-base anion exchange support that separates DNA oligomers according to negative charge. Because the PRP-X600 is a weak anion exchange (WAX) resin, the exchange capacity of the resin is pH dependent. Lowering the pH will reduce the binding of proteins, reducing the run time for a complete separation.
Change the mobile phase composition to alter the retention of DNA oligomers. Rapid gradient changes typically lower column efficiency; however, biomolecules run in this fashion on the PRP-X500 show very favorable separation efficiency with much shorter run times.
Hamilton RCX-10 and RCX-30
The Hamilton RCX-10 and RCX-30 carbohydrate analysis columns are designed for the isocratic or gradient separation of carbohydrates. The exchange capacity of the RCX-10/ RCX-30 is greater than that of the PRP-X100, leading to characteristics better suited for the separation of carbohydrates. Simple samples with two or three carbohydrates can be quickly separated isocratically, while more complex samples require gradient elution to fully resolve all the analytes of interest. When an isocratic method is used with a conductivity, refractive index, ultraviolet, or pulsed amperometric detector (PAD), mono and disaccharides such as glucose, fructose, sucrose and lactose can be quickly determined.
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