Bio SEC-3
Agilent Bio SEC-3 Columns
Agilent Bio SEC-3 columns are designed for high-resolution size exclusion chromatography of proteins, peptides, and biomolecular complexes—especially when pairing SEC with mass spectrometry (SEC-MS). Built on 3 µm, high-efficiency silica particles, Bio SEC-3 columns deliver sharp, symmetrical peaks and exceptional separation of monomers, aggregates, and fragments.
Engineered for modern bioanalysis, Bio SEC-3 columns are fully compatible with denaturing organic/aqueous mobile phases commonly used in SEC-MS workflows, providing reliable performance whether operating with low-salt MS-friendly conditions or higher-salt SEC buffers. Their mechanical robustness and optimized pore structure enable accurate, reproducible molecular-weight determinations across a wide range of biotherapeutic modalities—including mAbs, ADCs, peptides, and other next-generation biologics.
With full scalability from analytical to preparative dimensions, Bio SEC-3 columns support method development, routine QC, and high-throughput characterization on any LC/MS platform. For labs requiring sensitivity, reproducibility, and dependable molecular-size profiling, Agilent Bio SEC-3 delivers industry-leading performance and confidence.
Size Exclusion Chromatography (SEC)
A separation technique where molecules are resolved by size as they pass through a porous stationary phase. Larger molecules elute first, making SEC ideal for analyzing protein aggregates, fragments, and biomolecular purity.
SEC–MS (Size Exclusion Chromatography–Mass Spectrometry)
A hybrid technique coupling SEC with mass spectrometry to analyze protein mass, purity, and heterogeneity using mobile phases compatible with MS detection.
Pore Size
The diameter of pores in the stationary phase. Smaller pore sizes suit peptides and small proteins, while larger pores accommodate mAbs, ADCs, AAVs, and other high–molecular-weight biomolecules.
Hydrophilic Surface Chemistry
Surface modifications that minimize secondary interactions—critical in SEC to ensure proteins elute by size, not by charge or hydrophobic binding.
Aggregate Analysis
The quantification of protein dimers, trimers, and higher–order aggregates—a key quality attribute monitored in biotherapeutic development and QC.
What applications are Agilent Bio SEC-3 columns best suited for?
Bio SEC-3 columns are ideal for size-exclusion analysis of proteins, peptides, and biotherapeutics—especially when MS detection is required. They deliver fast, accurate quantitation of monomers, dimers, fragments, and aggregates.
Are Bio SEC-3 columns compatible with SEC-MS workflows?
Yes. Bio SEC-3 columns are optimized for organic/aqueous mobile phases typically used in SEC-MS, providing excellent stability and minimal carryover under both denaturing and native conditions.
Can Bio SEC-3 columns be scaled for preparative use?
Yes. The Bio SEC-3 platform offers seamless scalability—from analytical to preparative column dimensions—allowing the same separation selectivity and performance across purification workflows.
What mobile phase conditions are recommended?
Bio SEC-3 columns support both low-salt and high-salt SEC conditions, and are stable with the organic modifiers often required for SEC-MS, enabling flexible method development for diverse biotherapeutic samples.
Product List
| SKU | Name | LC Column Phase | Particle Size | Inner Diameter (ID) | Length |
|---|---|---|---|---|---|
SKU
5190-6856
|
Name
5190-6856 - Agilent SEC-3,300A,21.2x50mm
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
21.2 mm
|
Length
50 mm
|
SKU
5190-6855
|
Name
5190-6855 - Agilent SEC-3,150A,21.2x50mm
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
21.2 mm
|
Length
50 mm
|
SKU
5190-6854
|
Name
5190-6854 - Agilent SEC-3,100A,21.2x50mm
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
21.2 mm
|
Length
50 mm
|
SKU
5190-6852
|
Name
5190-6852 - Agilent SEC-3,300A,21.2x300mm
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
21.2 mm
|
Length
300 mm
|
SKU
5190-6851
|
Name
5190-6851 - Agilent SEC-3,150A,21.2x300mm
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
21.2 mm
|
Length
300 mm
|
SKU
5190-6850
|
Name
5190-6850 - Agient Bio SEC-3 100A, 21.2x300mm
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
21.2 mm
|
Length
300 mm
|
SKU
5190-6848
|
Name
5190-6848 - Agilent Bio SEC-3, 300A, 4.6x50mm, guard
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
4.6 mm
|
Length
50 mm
|
SKU
5190-6847
|
Name
5190-6847 - Agilent Bio SEC-3, 150A, 4.6x50mm, guard
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
4.6 mm
|
Length
50 mm
|
SKU
5190-6846
|
Name
5190-6846 - Agilent Bio SEC-3, 100A, 4.6x50mm, guard
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
4.6 mm
|
Length
50 mm
|
SKU
5190-2504
|
Name
5190-2504 - Agilent SEC-3, 100A, 4.6x150mm
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
4.6 mm
|
Length
150 mm
|
SKU
5190-2503
|
Name
5190-2503 - Bio SEC-3, 100A 4.6x300mm, 3um Column
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
4.6 mm
|
Length
300 mm
|
SKU
5190-2502
|
Name
5190-2502 - Agilent SEC-3, 100A, 7.8x150mm
|
LC Column Phase
SEC
|
Particle Size
3 um
|
Inner Diameter (ID)
7.8 mm
|
Length
150 mm
|