Blog

27th Mar 2024

Are Your Autosampler Vials Compromising Your Results?

Exploring the Impact of Vial Quality on HPLC and GC Performance In the world of chromatography, choosing the appropriate instrumentation and accessories is essential for achieving accurate and rel …

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5th Jan 2024

Revolutionizing Your Chromatography | The Power of Poroshell and more

Are you frustrated with increasing backpressures from your current HPLC column? Do you wish you could achieve UHPLC results with HPLC instrumentation? Choosing the proper tools for success is importan …

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3rd Jan 2024

Unlocking the Benefits of Agilent Bond Elut HLB SPE

Discover how Agilent Bond Elut HLB SPE products can revolutionize your sample preparation process and provide optimal results.Understanding the Importance of Sample Preparation Sample prepar …

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7th Nov 2023

EHS Compliance: Safely Manage HPLC Solvent Waste

Many of the solvents, and even samples, used in high performance liquid chromatography (HPLC) are hazardous and/or flammable. Because of this, it’s important to mitigate health and safety risks t …

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15th Aug 2023

How to Save Money and Increase Accuracy on Sample Prep

Laboratories can instantly increase throughput and improve their sample preparation accuracy with automation, but generally automation is only common in high throughput laboratories. But, what if th …

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9th Mar 2023

Syringe Filters for HPLC Sample Preparation: A Guide to Choosing the Right Filter for Consistent and Accurate Results

Sample preparation is a crucial step in high-performance liquid chromatography (HPLC). While sample preparation can vary depending on the type of application and level of interference a sample contain …

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13th Feb 2023

How to Choose the Right Septa for Your Autosampler Vial Closure

When performing LC and GC analysis, you’ll need something to store and transport your samples prior to injection. Chromatography vials are the most common vessel, but come in a variety of shapes and s …

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18th Sep 2022

What is the Smallest Sample Volume You Can Use in 96 Well Plates?

For high throughput laboratories, 96-well plates offer a number of advantages over chromatography vials such as cost savings and easy automation capabilities. Different well plate types serve differen …

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15th Sep 2022

What Sample Prep is Best for Vitamin D Analysis?

Low level testing of endogenous vitamin and hormone levels in biological samples is the driving force behind the need for more sensitive and specific analytical methods. One example is vitamin D analy …

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12th Jul 2022

Should You Use a Glass Pre-Filter Syringe Filter?

Should You be Using a Glass Pre-filter Syringe Filter?Proper sample filtration improves both the quality and consistency of analytical results, while also prolonging the lifetime of your instrument’s …

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2nd Jun 2022

Safety Waste Compliance in One Easy Step

Improper waste collection and handling of hazardous waste can put the people and property in a lab at risk. Long gone are the days of dumping HPLC solvent waste down the drain, but even experienced ch …

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2nd Jun 2022

Three Reasons You’ll Want to Switch to Agilent Capillary Tubing

Stainless steel tubing is the most popular material for HPLC and UHPLC due to it being compatible with many HPLC solvents, and able to withstand very high pressures. PEEK is the most common alternativ …

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12th May 2022

Revisiting ‘The Big Pain’

Throughout the pandemic, drug testing facilities were suddenly slammed with COVID-19 testing, meaning other testing (drug screening, etc.) was pushed to the wayside. This meant regular employee drug s …

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6th Mar 2022

How Many Injections Can I Get on My HPLC Column?

It depends! Many factors affect how many injections you can get on your column, including: Injecting “dirty” samplesFollowing HPLC Column manufacturers recommended protocol/operating conditionsUsing …

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11th Feb 2022

Method Transfer between Poroshell and Zorbax HPLC Columns

One of the primary benefits to using superficially porous particle (SPP) HPLC columns, as opposed to totally porous particles is the results yield the efficiency of sub-2-µm, fully porous columns, wit …

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11th Feb 2022

How do Stainless-Steel Fittings Differ from Polymer-Based Fittings?

Both stainless-steel and polymer-based fittings are quite common in HPLC, and there are pros and cons for each. Deciding which to use for your lab depends on the application and requirements of your m …

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9th Dec 2021

How to Connect a Syringe to HPLC Tubing

The need to connect a syringe to your LC pump to pull mobile phase through for priming (among other applications) is fairly common. We’ve seen some creative ways that people in the laboratory have con …

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15th Nov 2021

ASK BETHANY | Should I Use a Guard Column

HPLC ColumnsOne of the most important components in an LC system is the column, which is where analytes are separated out from a mixture for qualitative and quantitative analysis. HPLC columns need to …

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10th Nov 2021

An Essential Guide to HPLC Fittings for Your Laboratory

An Introduction to HPLC FittingsFittings are used any time tubing needs to be attached, adapted, or adjusted throughout the system, so creating good connections with fittings is extremely important fo …

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29th Sep 2021

A Sneak Peak into the Largest HPLC Industry in the World - Biotechnology Applications

Biotechnology is a broad term, and as a whole is one of the largest industries in the world. The biotechnology field is responsible for the success of food and agriculture, medicine, genetic resear …

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9th Sep 2021

Helpful Fluoropolymer Tubing Guide for Your HPLC Applications

What types of tubing are used in the laboratory? In liquid chromatography and other fluidic laboratory applications, you can choose from a wide variety of tubing materials, sizes, and other specific …

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29th Aug 2021

How Do You Dispose of HPLC Waste?

HPLC (High-Performance Liquid Chromatography) is used in many industries to separate complex mixtures of known or unknown analytes into individual components. With the use of internal or external s …

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11th Jul 2021

How To Make an HPLC Connection With PEEK Tubing

For fingertight fittings, the followings steps should be taken to make an HPLC connection: Slip the nut over the tubing that will go into the port.Slide the ferrule over the tubing, just below the nu …

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30th Sep 2020

Offline vs. Online Degassing of HPLC Solvents

Gas bubbles and outgassing can be detrimental to HPLC. Outgassing is created when there is more gas available than the HPLC solvent can absorb. The amount of gas liquids can absorb depends on many fac …

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28th Sep 2020

The Role of Chromatography in Cannabis Testing

The use of cannabis in the U.S. is growing rapidly as more states legalize its usage. Because of the newfound success in the industry, cannabis is now subject to mandatory testing in many states to en …

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28th Sep 2020

Inline Filters vs. Guard Columns for HPLC

Filters, such as inline filters, pre-column filters, and column guards, protect analytical columns from wear and premature failure within the HPLC system. The chromatographic separation of the HPLC co …

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26th Aug 2020

Reverse-Phase Liquid Chromatography vs. Hydrophilic Interaction Liquid Chromatography

Liquid Chromatography can be achieved through many methods including reverse-phase and hydrophilic interaction liquid chromatography (HILIC). Liquid chromatography is a form of column chromatography t …

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16th Jul 2020

Chromatography Identifies Clinical Classifiers of COVID-19

The COVID-19 pandemic has created unprecedented struggles across the world due to symptom variability and high spread rate. As COVID-19 continues to surge through the U.S., there is an increasing need …

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23rd Jan 2020

Capillary Tubing Selection Guide for Agilent HPLC Systems

Chrom Tech is constantly working to make shopping easier for consumers. Replacing the various capillary tubing connections in your HPLC can be intimidating and confusing.In our latest efforts, we put …

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20th Nov 2019

Reduce PFAS Background Interference and Contamination

According to the EPA, PFAS are persistent in the environment and human body, and exposure to these compounds can lead to adverse health effects. Per/Polyfluoroalkyl substances (PFAS), also known as pe …

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