In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples. The stronger the positive (+) charge on the sample, the stronger it will be attracted to the negative charge on the stationary phase, and thus the longer it will take to elute. The mobile phase is an aqueous buffer, where both pH and ionic strength are used to control elution time. Ion chromatography can employ harsh conditions requiring mobile phases that are at very high pH limits (> 11). Temperatures well above the normal operating conditions where silica materials fail can also be used.
Hamilton offers five polymeric packing materials for anion exchange separations.
Hamilton PRP-X200 cation exchange HPLC columns are designed for rapid, high resolution separation of alkali and alkaline earth metals. The alkali metals and ammonium are completely resolved in less than five minutes, and the alkaline earth cations separate in under four minutes. Since the mobile phase conditions are different and unique for each of the groups of cations, interferences between these groups are eliminated.
The PRP-X400 column provides a fast separation for glyphosate and its metabolites. The exchange capacity of the PRP-X400 is greater than that of the PRP-X200, leading to characteristics better suited for the separation of glyphosate. It also performs well in other separations, such as inositol and sugar alcohols.
The PRP-X800 is a polymeric cation exchange column functionalized with itaconic acid that performs the isocratic separation of mono and divalent cations such as lithium, sodium, ammonium, potassium, magnesium and calcium. The column offers excellent durability, is stable to any concentration organic solvent, and enables dynamic control of exchange capacity.
HC-40 columns separate compounds through size exclusion. The 4% cross-linked HC-40 uses size exclusion as the primary mechanism of separation. The larger carbohydrate oligomers elute first while the smaller di- and monosaccharaides elute later.
HC-75 columns separate compounds through ligand exchange. The 8% cross-linked HC-75 uses ligand exchange as the primary mechanism of separation. The different forms of the HC-75 (Hydrogen, Calcium and Lead) each provide a unique selectivity for separating varying types of charged analytes based on electronegativity toward the counterion. The larger carbohydrate oligomers elute first while the smaller di- and monosaccharaides elute later.
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