Cyclodex-B

Cyclodex-B column

Agilent Cyclodex-B Chiral GC Columns

Agilent J&W Cyclodex-B GC columns utilize a 10.5% β-cyclodextrin stationary phase engineered for high-performance chiral separations. This cyclodextrin-based phase forms selective inclusion complexes with enantiomers, enabling efficient resolution of chiral compounds across a wide range of flavors, fragrances, essential oils, environmental contaminants, and specialty chemicals—without the need for chiral-specific derivatization.

Cyclodex-B delivers outstanding peak shapes, strong enantiomeric selectivity, and broad applicability, making it an excellent choice for laboratories requiring reliable separation of structurally similar enantiomers. Its sensitivity, precision, and reproducibility support confident quantitation and enantiomeric purity assessment in both routine QC workflows and advanced R&D environments. Whether applied to aroma-active compounds, chiral markers, or trace-level optical isomers, Cyclodex-B provides the stability and performance needed for demanding chiral analyses.
Key Definitions â–¸
Cyclodex-B
A chiral GC column containing a 10.5% β-cyclodextrin stationary phase that enables high-resolution enantiomeric separations without requiring derivatization. Well-suited for flavors, fragrances, essential oils, and specialty chemical analysis.
β-Cyclodextrin Inclusion Complexing
A mechanism in which β-cyclodextrin forms temporary host–guest complexes with analytes. Because enantiomers interact differently with the cyclodextrin cavity, the column can separate them based on subtle stereochemical differences.
Enantiomeric Purity
The relative ratio of two enantiomers in a sample. Cyclodex-B enables accurate determination of enantiomeric purity for quality control, authenticity verification, and regulatory compliance.
Chiral-Specific Derivatization
A chemical modification technique used to introduce chirality into analytes prior to GC analysis. Cyclodex-B eliminates the need for such derivatization by providing direct chiral resolution through its β-cyclodextrin phase.
Frequently Asked Questions â–¸
What types of chiral compounds are best analyzed on Cyclodex-B?
Cyclodex-B excels at separating chiral compounds commonly found in flavors, fragrances, essential oils, agrochemicals, and specialty chemicals. It is particularly effective for resolving enantiomeric pairs that influence aroma, sensory character, or biological activity.
Why is chiral derivatization not required for Cyclodex-B?
The β-cyclodextrin phase forms selective inclusion complexes with each enantiomer. Because these interactions differ based on stereochemistry, Cyclodex-B achieves direct chiral separation without the need for pre-column derivatization.
What contributes to the excellent peak shapes observed with Cyclodex-B?
Cyclodex-B’s optimized β-cyclodextrin loading and high inertness minimize adsorption and active-site interactions, resulting in sharp, symmetric peaks even for challenging polar chiral analytes.
Can Cyclodex-B be solvent rinsed to extend lifetime?
No. Cyclodex-B is a non-bonded phase and should not be solvent-rinsed. Its performance and lifetime are best maintained through proper inlet maintenance and clean sample preparation.