CycloSil-B

CycloSil-B column

Agilent CycloSil-B Chiral GC Columns

Agilent J&W CycloSil-B GC columns feature a modified dimethylsilyl β-cyclodextrin stationary phase designed to deliver enhanced enantioselectivity for a broad range of chiral analytes. This specialized cyclodextrin phase interacts selectively with enantiomers, enabling high-resolution separation of compounds commonly found in flavors, fragrances, essential oils, and natural product chemistry. CycloSil-B is particularly effective for chiral γ-lactones, terpenes, and other aroma-active molecules—without the need for chiral-specific derivatization.

The unique inclusion-complex mechanism of the β-cyclodextrin phase provides strong chiral recognition, making CycloSil-B an excellent choice for laboratories requiring precise characterization of enantiomeric purity, authenticity, or sensory contribution in complex mixtures. Its high selectivity supports both routine quality control and advanced R&D workflows across food, fragrance, and specialty chemical applications.

CycloSil-B is a non-bonded, non-crosslinked phase, and therefore solvent rinsing is not recommended. With proper inlet maintenance and clean sample handling, the column delivers stable retention, excellent precision, and reliable long-term performance for demanding chiral GC analyses.
Key Definitions â–¸
CycloSil-B
A chiral GC stationary phase based on modified dimethylsilyl β-cyclodextrin, providing strong enantioselectivity for γ-lactones, terpenes, essential oils, and other chiral volatiles without requiring chemical derivatization.
β-Cyclodextrin Phase
A cyclic oligosaccharide structure that forms inclusion complexes with analytes. In chiral GC, β-cyclodextrin selectively interacts with enantiomers, enabling high-resolution separation of mirror-image molecules.
Enantiomeric Separation
The chromatographic resolution of two mirror-image molecules (enantiomers) that have identical physical properties but differ in biological or sensory effects. CycloSil-B enables this separation via selective host–guest interactions.
Non-Bonded Phase
A stationary phase not chemically attached or crosslinked to the silica surface. Non-bonded phases, including CycloSil-B, offer strong selectivity but cannot be solvent-rinsed, making clean sample preparation essential for long-term performance.
Frequently Asked Questions â–¸
What types of compounds are best separated on CycloSil-B?
CycloSil-B is ideal for separating chiral γ-lactones, terpenes, essential oils, and other aroma-active volatiles commonly found in flavors, fragrances, and natural product chemistry. It provides reliable enantiomeric resolution without requiring derivatization.
How does the β-cyclodextrin phase enable chiral separations?
The modified β-cyclodextrin forms temporary inclusion complexes with analytes. Because each enantiomer interacts slightly differently with the cyclodextrin cavity, their retention times diverge, allowing high-resolution chiral separation.
Can CycloSil-B be solvent rinsed?
No. CycloSil-B is a non-bonded, non-crosslinked phase and should not be solvent-rinsed. Clean injection techniques and routine inlet maintenance help preserve performance and retention stability.
What industries commonly use CycloSil-B chiral GC columns?
CycloSil-B is widely used in the flavors, fragrances, essential oil, environmental, and specialty chemical sectors—anywhere enantiomeric purity or chiral compound identification is needed for quality, authenticity, or regulatory compliance.