Poroshell Peptide Mapping

Agilent InfinityLab Poroshell 120 AdvanceBio Peptide Mapping

The InfinityLab Poroshell 120 AdvanceBio Peptide Mapping columns are purpose-built for high-confidence peptide mapping, sequence confirmation, and post-translational modification (PTM) analysis. Engineered with 2.7 µm superficially porous particles and optimized bonded phases, these columns deliver outstanding resolution, sharper peaks, and improved sensitivity for complex peptide mixtures.

Every batch of AdvanceBio Peptide Mapping media is QA-tested with a rigorous multi-peptide standard, ensuring consistent selectivity, retention, and chromatographic performance across lots. This guarantees reliable identification of key peptides—critical for biopharmaceutical characterization, biosimilar comparison, and QC workflows.

Designed for robustness and ease of use, Poroshell 120 AdvanceBio columns provide fast, reproducible separations with lower backpressure, enabling compatibility with both traditional HPLC and modern UHPLC systems. Their high efficiency makes them ideal for workflows such as peptide mapping, PTM profiling, peptide impurity analysis, and biomarker discovery.

Whether optimizing a regulated biopharma method or developing new analytical workflows, Agilent InfinityLab Poroshell 120 AdvanceBio Peptide Mapping columns offer the performance, reproducibility, and confidence required for today’s complex peptide analyses.

Agilent InfinityLab Poroshell 120 AdvanceBio Peptide Mapping — Phase Description ▸
Poroshell 120 AdvanceBio Peptide Mapping C18
SPP (solid-core) • 120 Å • Optimized for peptide mapping • Bio-inert surface • High coverage
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Poroshell 120 AdvanceBio Peptide Mapping C18 is engineered for high-resolution reversed-phase separations of enzymatically digested peptides. Built on Agilent’s solid-core (SPP) particle platform, it delivers UHPLC-level efficiency with manageable backpressure—ideal for peptide mapping workflows in biopharmaceutical development and QC.

When to Choose This Phase
  • Tryptic peptide mapping of monoclonal antibodies
  • Sequence confirmation and PTM analysis
  • LC/MS-based peptide characterization
  • High-coverage, high-resolution gradient separations
How It Compares

Compared to conventional fully porous 3–5 µm C18 columns, the Poroshell 120 SPP design improves peak capacity and reduces diffusion broadening—critical for resolving complex peptide mixtures. It enables faster cycle times without sacrificing resolution.

Key Specifications
Separation ModeReversed Phase (C18)
Particle TechnologySuperficially Porous Particle (SPP / solid-core)
Pore Size120 Å
Application FocusPeptide mapping & protein digest analysis
Detection CompatibilityUV, FLD, LC/MS
Typical WorkflowTryptic digests of mAbs and recombinant proteins

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SKU Name LC Column Phase Particle Size Inner Diameter (ID) Length
SKU
863600-911
Name
863600-911 - AdvanceBio Peptide Mapping, 1.0 x 150 mm
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
1 mm
Length
150 mm
SKU
655950-302
Name
655950-302 - AdvanceBio, Peptide Mapping, 3.0 mm, 100 mm, 2.7 um column
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
3 mm
Length
100 mm
SKU
5065-9946
Name
5065-9946 - AdvanceBio Peptide Mapping, 0.3 x 50mm, 2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
0.3 mm
Length
50 mm
SKU
5065-9925
Name
5065-9925 - AdvanceBio Peptide Mapping, 0.075x150mm
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
0.075 mm
Length
150 mm
SKU
850750-911
Name
850750-911 - Bio-C Peptide Map, 4.6 mm Guard
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
4.6 mm
Length
5 mm
SKU
853750-911
Name
853750-911 - Bio-C Peptide Map, 3.0 mm Guard
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
3 mm
Length
5 mm
SKU
851725-911
Name
851725-911 - Bio-C Peptide Map, 2.1 mm Guard
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
Length
5 mm
SKU
655750-902
Name
655750-902 - AdvanceBio Peptide Map, 2.1x100mm,2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
Length
100 mm
SKU
653950-902
Name
653950-902 - Bio-C Peptide Map, 4.6x150mm, 2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
4.6 mm
Length
150 mm
SKU
653950-302
Name
653950-302 - Bio-C Peptide Map, 3.0x150mm, 2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
3 mm
Length
150 mm
SKU
653750-902
Name
653750-902 - Bio-C Peptide Map, 2.1x150mm,2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
Length
150 mm
SKU
651750-902
Name
651750-902 - Bio-C Peptide Map, 2.1x250mm, 2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
Length
250 mm
Key Definitions ▸
Peptide Mapping
A reversed-phase LC workflow in which proteins are enzymatically digested (commonly with trypsin) and the resulting peptides are separated to confirm sequence coverage, detect post-translational modifications (PTMs), and assess product consistency.
Superficially Porous Particles (SPP)
Also known as core–shell particles, SPP technology uses a solid core with a thin porous shell. This reduces diffusion distance, improves peak capacity, and enables high-resolution separations at moderate backpressure—ideal for complex peptide digests.
C18 Reversed-Phase Selectivity
A hydrophobic stationary phase that separates peptides based on hydrophobicity. C18 is the preferred chemistry for peptide mapping because it provides strong retention, high peak capacity, and compatibility with LC/MS workflows.
120 Å Pore Size
A pore diameter optimized for peptide separations. It provides efficient mass transfer for peptides generated from protein digests, balancing retention strength and chromatographic efficiency.
Post-Translational Modifications (PTMs)
Chemical modifications occurring after protein synthesis, such as oxidation, deamidation, glycosylation, or phosphorylation. Peptide mapping methods are designed to detect and quantify these modifications.
Sequence Coverage
The percentage of a protein’s amino acid sequence confirmed by detected peptides. High sequence coverage improves confidence in identity confirmation and structural integrity assessments.
Frequently Asked Questions ▸
What is the AdvanceBio Peptide Mapping column used for?
AdvanceBio Peptide Mapping columns are designed for reversed-phase separation of enzymatically digested proteins. They are commonly used for sequence confirmation, post-translational modification (PTM) analysis, and structural characterization of biotherapeutics.
Why is C18 the preferred phase for peptide mapping?
C18 reversed-phase chemistry provides strong hydrophobic retention, high peak capacity, and excellent gradient compatibility for peptide separations. It enables reliable resolution of complex tryptic digests and supports LC/MS workflows.
How does Poroshell solid-core technology improve peptide separations?
The superficially porous (SPP) particle design reduces diffusion distance, producing sharper peaks and higher resolution compared to traditional fully porous particles. This is especially important when resolving complex peptide mixtures with many closely eluting species.
Are these columns compatible with LC/MS detection?
Yes. AdvanceBio Peptide Mapping columns are fully compatible with LC/MS workflows, making them ideal for peptide identification, PTM analysis, and high-resolution mass-based characterization.
What applications benefit most from AdvanceBio Peptide Mapping columns?
These columns are widely used for biopharmaceutical characterization, biosimilar comparison, stability studies, and regulatory submissions where high sequence coverage, reproducibility, and reliable PTM detection are required.