Poroshell Peptide Mapping

Agilent InfinityLab Poroshell 120 AdvanceBio Peptide Mapping

The InfinityLab Poroshell 120 AdvanceBio Peptide Mapping columns are purpose-built for high-confidence peptide mapping, sequence confirmation, and post-translational modification (PTM) analysis. Engineered with 2.7 µm superficially porous particles and optimized bonded phases, these columns deliver outstanding resolution, sharper peaks, and improved sensitivity for complex peptide mixtures.

Every batch of AdvanceBio Peptide Mapping media is QA-tested with a rigorous multi-peptide standard, ensuring consistent selectivity, retention, and chromatographic performance across lots. This guarantees reliable identification of key peptides—critical for biopharmaceutical characterization, biosimilar comparison, and QC workflows.

Designed for robustness and ease of use, Poroshell 120 AdvanceBio columns provide fast, reproducible separations with lower backpressure, enabling compatibility with both traditional HPLC and modern UHPLC systems. Their high efficiency makes them ideal for workflows such as peptide mapping, PTM profiling, peptide impurity analysis, and biomarker discovery.

Whether optimizing a regulated biopharma method or developing new analytical workflows, Agilent InfinityLab Poroshell 120 AdvanceBio Peptide Mapping columns offer the performance, reproducibility, and confidence required for today’s complex peptide analyses.

Key Definitions

Reversed-Phase Chromatography (RP)

A hydrophobic separation mode using C18, C8, phenyl, and similar phases. Ideal for small molecules, peptides, and biomolecules across pharmaceutical and environmental workflows.

Superficially Porous Particles (SPP)

Also known as “core-shell” particles. Provide UHPLC-like efficiency with lower backpressure, enabling faster, higher-resolution separations on standard LC systems.

Fully Porous Particles (FPP)

Traditional particle design used in many ZORBAX, Polaris, and Pursuit column families. Offers broad selectivity, robust durability, and high loading capacity.

Stationary Phase

The surface chemistry bonded to the silica or core-shell particle—such as C18, C8, Phenyl, CN, Amide, or HILIC—that controls selectivity, retention, and peak shape.

HILIC (Hydrophilic Interaction Chromatography)

A separation mode ideal for polar analytes using hydrophilic stationary phases and high-organic mobile phases, providing strong retention where reversed-phase fails.

Frequently Asked Questions

What types of analyses is this column designed for?

This column supports high-resolution peptide mapping, enabling confident identification of key peptides in complex biotherapeutic digests. It is ideal for QC, characterization, comparability studies, and stability-indicating workflows.

What makes AdvanceBio peptide mapping media highly reproducible?

Each batch is QA-tested using a rigorous peptide mixture, ensuring consistent retention, peak shape, and selectivity so analysts can identify critical quality attributes across batches.

Is this column suitable for LC/MS workflows?

Yes. Its optimized particle design and controlled selectivity support excellent sensitivity, making it fully compatible with LC/MS detection for peptide mapping and biotherapeutic analysis.

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SKU Name LC Column Phase Particle Size Inner Diameter (ID) Length
SKU
863600-911
Name
863600-911 - AdvanceBio Peptide Mapping, 1.0 x 150 mm
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
1 mm
Length
150 mm
SKU
655950-302
Name
655950-302 - AdvanceBio, Peptide Mapping, 3.0 mm, 100 mm, 2.7 um column
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
3 mm
Length
100 mm
SKU
5065-9946
Name
5065-9946 - AdvanceBio Peptide Mapping, 0.3 x 50mm, 2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
0.3 mm
Length
50 mm
SKU
5065-9925
Name
5065-9925 - AdvanceBio Peptide Mapping, 0.075x150mm
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
0.075 mm
Length
150 mm
SKU
850750-911
Name
850750-911 - Bio-C Peptide Map, 4.6 mm Guard
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
4.6 mm
Length
5 mm
SKU
853750-911
Name
853750-911 - Bio-C Peptide Map, 3.0 mm Guard
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
3 mm
Length
5 mm
SKU
851725-911
Name
851725-911 - Bio-C Peptide Map, 2.1 mm Guard
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
Length
5 mm
SKU
655750-902
Name
655750-902 - AdvanceBio Peptide Map, 2.1x100mm,2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
Length
100 mm
SKU
653950-902
Name
653950-902 - Bio-C Peptide Map, 4.6x150mm, 2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
4.6 mm
Length
150 mm
SKU
653950-302
Name
653950-302 - Bio-C Peptide Map, 3.0x150mm, 2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
3 mm
Length
150 mm
SKU
653750-902
Name
653750-902 - Bio-C Peptide Map, 2.1x150mm,2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
Length
150 mm
SKU
651750-902
Name
651750-902 - Bio-C Peptide Map, 2.1x250mm, 2.7um
LC Column Phase
C18
Particle Size
2.7 um
Inner Diameter (ID)
2.1 mm
Length
250 mm