Organic Acid Analysis

Concise Separations Columns for Organic Acid Analysis

  • Wide pH stability from 0 to 14 for robust, flexible method development
  • Thermally stable at temperatures up to 90 °C
  • Consistent performance maintained through numerous injections with proper sample prep, clean system operation, and guard column protection
  • Isocratic separations enabled by simple dilute acid—no gradients required
  • Eliminates the need for costly organic solvents and solvent waste handling
  • Eluent acts as a self-regenerating cleaning solution without degrading column chemistry

Concise Separations organic acid columns are engineered for high-efficiency, reproducible separation of a wide variety of organic acids using simple, low-cost eluents. These columns rely on well-defined acid–base interactions within the stationary phase, enabling predictable retention across a broad pH range and delivering excellent peak shape for even highly polar, strongly retained acids.

Eluent Effect: By precisely controlling the true strength of the acidic mobile phase, analysts can fine-tune compound retention relative to each analyte’s pKa. A stronger (more acidic) eluent increases retention times and enhances the separation of compounds with closely spaced acid dissociation constants. This tunable behavior allows the column to deliver optimized selectivity without gradients or complex solvent systems, while maintaining exceptional robustness and long lifetime.

Key Definitions ▸
Eluent Strength
The acidic concentration of the mobile phase, which directly influences organic acid retention. Stronger acidic eluents increase analyte interaction with the stationary phase, extending retention times and improving separation selectivity.
pH Stability Range (0–14)
The full spectrum of acidic to basic conditions the column can withstand without degradation, allowing aggressive cleaning, flexible method development, and reliable retention across diverse acid chemistries.
Isocratic Separation
A chromatographic method performed with a constant mobile phase composition. Concise Separations organic acid columns achieve strong separation power using only dilute acid, eliminating gradient complexity.
pKa–Retention Relationship
The influence of an analyte’s acid dissociation constant (pKa) on its chromatographic behavior. Adjusting eluent strength shifts the ionization state and retention of organic acids, enabling fine-tuned selectivity.
Self-Regenerating Eluent
A simple dilute acid mobile phase that continuously cleans the stationary phase during operation, reducing fouling, extending column lifetime, and eliminating the need for high-cost organic solvents.
Frequently Asked Questions ▸

Why are Concise Separations columns ideal for organic acid analysis?

These columns offer full pH stability (0–14), high thermal tolerance, and strong retention control using simple dilute acid eluents—delivering reliable separations without gradients or expensive solvents.

Do I need organic solvents for these methods?

No. The mobile phase is typically a simple dilute acid, which eliminates the need for expensive organic solvents and reduces waste disposal costs.

How does eluent strength affect retention times?

Increasing the acidity (strength) of the eluent increases analyte interaction with the stationary phase, resulting in longer retention times and improved separation of closely related organic acids.