Aqueous GPC/SEC Columns

HPLC Columns for Aqueous GPC and SEC Analyses

Chrom Tech supplies the full portfolio of Agilent aqueous GPC and SEC columns designed for precise molecular weight characterization of polymers, plastics, biomolecules, and hydrophilic materials. The industry-leading PL aquagel-OH and PolarGel families deliver reliable separations in water, buffers, and polar solvent systems, making them ideal for environmentally friendly and biocompatible workflows.

Engineered for stability, low swelling, and exceptional batch-to-batch reproducibility, these columns offer a wide range of pore sizes, particle sizes, and hardware formats to match any analytical requirement—from routine QC to advanced research on complex polymer architectures. Their optimized hydrophilic stationary phases help ensure accurate calibration curves, consistent retention, and dependable long-term performance.

Whether you are characterizing natural polymers, synthetic hydrogels, polysaccharides, water-soluble plastics, or other macromolecules, Agilent’s aqueous GPC/SEC solutions provide the resolution, durability, and flexibility needed for high-confidence results across modern polymer labs.

Key Definitions

Gel Permeation Chromatography (GPC)

A size-based separation technique where larger molecules elute earlier because they are excluded from the pores of the stationary phase. Commonly used for polymer molecular-weight distribution analysis.

Size Exclusion Chromatography (SEC)

A liquid-phase form of size-based separation used for macromolecules in aqueous or polar solvents, including biopolymers, polysaccharides, and water-soluble synthetic polymers.

Pore Size

The diameter of the pores in the stationary phase. Larger pores allow high-molecular-weight species to access the stationary phase, enabling accurate SEC/GPC characterization of large polymers.

Aqueous GPC Media

Polymer or silica-based stationary phases engineered for compatibility with water, buffers, and polar solvents. Used for hydrophilic polymers and biopolymers that cannot tolerate organic solvent systems.

Polar Gel Media (e.g., PolarGel)

Hydrophilic, solvent-resistant phases designed for reproducible separations in water, mixed aqueous systems, or high-salt environments—ideal for polymers that swell or degrade in organic-only conditions.

Frequently Asked Questions

What types of samples can be analyzed using aqueous GPC/SEC columns?

Aqueous GPC/SEC columns are designed for polymers, biomolecules, polysaccharides, water-soluble plastics, and other macromolecules analyzed in water, buffers, or polar solvents. They offer reliable size-based separation for a wide range of hydrophilic materials.

What is the benefit of using PL aquagel-OH or PolarGel columns?

PL aquagel-OH and PolarGel columns provide excellent stability in aqueous and mixed solvent systems, low nonspecific adsorption, and highly reproducible molecular weight determinations—ideal for routine QC and advanced research applications.

Are these aqueous GPC/SEC columns compatible with standard HPLC systems?

Yes. These columns are compatible with conventional HPLC and GPC/SEC systems using aqueous mobile phases. Multiple dimensions, porosities, and hardware formats allow easy integration into existing workflows.

Product List

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SKU Name Type Length Inner Diameter (ID) Particle Size
SKU
PL2070-6001
Name
PL2070-6001 - PEG nominal Mp 1.5k 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2084-2001
Name
PL2084-2001 - PEO nominal Mp 1.5m 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2084-1001
Name
PL2084-1001 - PEO nominal Mp 1m 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2070-5001
Name
PL2070-5001 - PEG nominal Mp 1k 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2084-0001
Name
PL2084-0001 - PEO nominal Mp 700k 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2083-9001
Name
PL2083-9001 - PEO nominal Mp 500k 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2070-4001
Name
PL2070-4001 - PEG nominal Mp 600 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2083-8001
Name
PL2083-8001 - PEO nominal Mp 300k 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2083-7001
Name
PL2083-7001 - PEO nominal Mp 200k 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2070-3001
Name
PL2070-3001 - PEG nominal Mp 400 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2083-6001
Name
PL2083-6001 - PEO nominal Mp 130k 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size
SKU
PL2070-2001
Name
PL2070-2001 - PEG nominal Mp 194 1g
Type
Standard
Length
Inner Diameter (ID)
Particle Size