PLRP-S
Agilent PLRP-S Reversed-Phase Columns
Agilent PLRP-S columns are engineered using fully polymeric particles that deliver exceptional chemical, thermal, and mechanical stability across the full pH range—making them a trusted choice for the most demanding bioanalytical and industrial separations. Unlike silica-based phases, PLRP-S media contain no silanols, no heavy-metal contaminants, and no bonded alkyl ligands, ensuring highly reproducible retention and robust long-term performance.
Designed for maximum versatility, PLRP-S columns are available in an extensive selection of pore sizes from 100 Å to 4000 Å, allowing analysts to optimize separations for small molecules, peptides, proteins, oligonucleotides, antibody fragments, viral particles, and large biomolecular complexes. Each pore size maintains identical surface chemistry, enabling consistent selectivity across analytical, semiprep, and preparative formats.
With compliance to USP L21 and unmatched ruggedness under elevated temperature, extreme pH, and aggressive cleaning conditions, PLRP-S is widely used across pharmaceutical, biopharma, environmental, polymer, and materials-science laboratories. These polymeric particles excel in reversed-phase workflows where silica-based columns fail due to hydrolysis or limited pH stability.
Chrom Tech proudly supplies the full portfolio of Agilent PLRP-S columns, including analytical, high-resolution, and preparative configurations for scalable purification and comprehensive biomolecule characterization.
Polymeric Reversed-Phase (PLRP-S)
A fully polymeric, styrene–divinylbenzene stationary phase used for reversed-phase separations. Unlike silica, PLRP-S is free from silanols and metal impurities, offering extremely consistent retention and long lifetime across harsh pH and temperature conditions.
Pore Size
PLRP-S offers pore sizes from 100Å to 4000Å, allowing optimized separations for small molecules, peptides, proteins, antibody fragments, and large nucleic acids or complexes.
Hydrophobic Interaction (No Bonded Phase)
Unlike silica columns that require C18 or other bonded ligands, PLRP-S uses inherently hydrophobic polymeric beads. This eliminates variability from bonded-phase chemistry and ensures extremely consistent method performance.
Chemical & Thermal Stability
PLRP-S withstands aggressive solvents, high temperatures, and a wide pH range without degradation—ideal for biomolecules that require denaturing conditions, high-temperature elution, or repeated cleaning cycles.
USP L21 Classification
Indicates a polymeric reversed-phase packing used for applications requiring high durability and compatibility with biomolecules, pharmaceuticals, and large analytes.
What makes PLRP-S columns different from silica-based columns?
PLRP-S columns use highly durable polymeric particles instead of silica, eliminating silanols and metal impurities. This provides excellent chemical and thermal stability, broader pH capability, and more reproducible retention for biomolecules and complex samples.
Which applications benefit most from PLRP-S columns?
PLRP-S is widely used for biomolecule separations, including peptides, proteins, oligonucleotides, polynucleotides, and large complexes. Its stability also makes it ideal for pharmaceutical, environmental, food, chemical, and materials-science workflows.
How do I select the correct pore size for my sample?
Choose 100–300 Å pores for small molecules and peptides, mid-range pores for proteins, and 1000–4000 Å pores for very large biomolecules, aggregates, and polynucleotides. Each pore size uses the same chemistry for consistent method transfer.
Can PLRP-S columns be used for preparative purification?
Yes. PLRP-S columns are available in analytical and preparative formats, making them suitable for both discovery-scale separations and high-load purifications requiring consistent, reproducible performance.
Product List
| SKU | Name | LC Column Phase | Particle Size | Inner Diameter (ID) | Length |
|---|---|---|---|---|---|
SKU
PL1912-3300
|
Name
PL1912-3300 - PLRP-S 100A 3uM 150X2.1MM
|
LC Column Phase
DVB
|
Particle Size
3 um
|
Inner Diameter (ID)
2.1 mm
|
Length
150 mm
|
SKU
PL1912-1803
|
Name
PL1912-1803 - PLRP-S 4000A 8uM 50X2.1MM
|
LC Column Phase
DVB
|
Particle Size
8 um
|
Inner Diameter (ID)
2.1 mm
|
Length
50 mm
|
SKU
PL1912-1802
|
Name
PL1912-1802 - PLRP-S 1000A 8uM 50X2.1MM
|
LC Column Phase
DVB
|
Particle Size
8 um
|
Inner Diameter (ID)
2.1 mm
|
Length
50 mm
|
SKU
PL1612-1801
|
Name
PL1612-1801 - PLRP-S GUARD CARTRIDGES 5X3MM (X2)
|
LC Column Phase
DVB
|
Particle Size
5 um
|
Inner Diameter (ID)
3 mm
|
Length
5 mm
|
SKU
PL1912-1801
|
Name
PL1912-1801 - PLRP-S 300A 8uM 50X2.1MM
|
LC Column Phase
DVB
|
Particle Size
8 um
|
Inner Diameter (ID)
2.1 mm
|
Length
50 mm
|
SKU
PL1912-1503
|
Name
PL1912-1503 - PLRP-S 4000A 5uM 50X2.1MM
|
LC Column Phase
DVB
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
50 mm
|
SKU
PL1912-1502
|
Name
PL1912-1502 - PLRP-S 1000A 5UM 50X2.1MM
|
LC Column Phase
DVB
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
50 mm
|
SKU
PL1912-1501
|
Name
PL1912-1501 - PLRP-S 300A 5uM 50X2.1MM
|
LC Column Phase
DVB
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
50 mm
|
SKU
PL1912-1500
|
Name
PL1912-1500 - PLRP-S 100A 5uM 50X2.1MM
|
LC Column Phase
DVB
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
50 mm
|
SKU
PL1912-5801
|
Name
PL1912-5801 - PLRP-S 300A 8uM 250X2.1MM
|
LC Column Phase
DVB
|
Particle Size
8 um
|
Inner Diameter (ID)
2.1 mm
|
Length
250 mm
|
SKU
PL1912-1301
|
Name
PL1912-1301 - PLRP-S 300A 3uM 50X2.1MM
|
LC Column Phase
DVB
|
Particle Size
3 um
|
Inner Diameter (ID)
2.1 mm
|
Length
50 mm
|
SKU
PL1512-3301
|
Name
PL1512-3301 - PLRP-S 300A 3uM 150X4.6MM
|
LC Column Phase
DVB
|
Particle Size
3 um
|
Inner Diameter (ID)
4.6 mm
|
Length
150 mm
|