PLRP-S

Agilent PLRP-S Reversed-Phase Columns

Agilent PLRP-S columns are engineered using fully polymeric particles that deliver exceptional chemical, thermal, and mechanical stability across the full pH range—making them a trusted choice for the most demanding bioanalytical and industrial separations. Unlike silica-based phases, PLRP-S media contain no silanols, no heavy-metal contaminants, and no bonded alkyl ligands, ensuring highly reproducible retention and robust long-term performance.

Designed for maximum versatility, PLRP-S columns are available in an extensive selection of pore sizes from 100 Ã… to 4000 Ã…, allowing analysts to optimize separations for small molecules, peptides, proteins, oligonucleotides, antibody fragments, viral particles, and large biomolecular complexes. Each pore size maintains identical surface chemistry, enabling consistent selectivity across analytical, semiprep, and preparative formats.

With compliance to USP L21 and unmatched ruggedness under elevated temperature, extreme pH, and aggressive cleaning conditions, PLRP-S is widely used across pharmaceutical, biopharma, environmental, polymer, and materials-science laboratories. These polymeric particles excel in reversed-phase workflows where silica-based columns fail due to hydrolysis or limited pH stability.

Chrom Tech proudly supplies the full portfolio of Agilent PLRP-S columns, including analytical, high-resolution, and preparative configurations for scalable purification and comprehensive biomolecule characterization.

Agilent PLRP-S Reversed-Phase — Phase Description ▸
PLRP-S DVB (Styrene-Divinylbenzene)
Polymeric RP • PS-DVB matrix • pH 1–13 stability • High temperature tolerant • No silica
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Agilent PLRP-S is a fully polymeric reversed-phase column built on a cross-linked styrene-divinylbenzene (PS-DVB) matrix. Unlike silica-based C18 phases, PLRP-S offers exceptional chemical stability across a wide pH range, making it ideal for aggressive mobile phases and high-temperature applications.

When to Choose This Phase
  • High-pH or extreme pH separations (up to pH 13)
  • High-temperature protein or polymer analysis
  • Peptide, oligonucleotide, or intact protein work under harsh conditions
  • Applications where silica dissolution is a concern
How It Compares

Compared to silica-based C18 columns, PLRP-S provides broader pH tolerance and improved stability in basic or aggressive solvents. While it may exhibit different selectivity due to its polymeric surface chemistry, it is often selected when method durability outweighs traditional silica-based behavior.

Key Specifications
Separation ModeReversed Phase (Polymeric)
Stationary PhaseStyrene-Divinylbenzene (PS-DVB)
Particle TypeFully porous polymeric particles
pH Range1 – 13
Temperature CapabilityHigh temperature tolerant (method dependent)
Silica ContentNone (fully polymeric)
Typical ApplicationsProteins, peptides, polymers, oligonucleotides

Product List

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SKU Name LC Column Phase Particle Size Inner Diameter (ID) Length
SKU
PL1912-3300
Name
PL1912-3300 - PLRP-S 100A 3uM 150X2.1MM
LC Column Phase
DVB
Particle Size
3 um
Inner Diameter (ID)
2.1 mm
Length
150 mm
SKU
PL1912-1803
Name
PL1912-1803 - PLRP-S 4000A 8uM 50X2.1MM
LC Column Phase
DVB
Particle Size
8 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
PL1912-1802
Name
PL1912-1802 - PLRP-S 1000A 8uM 50X2.1MM
LC Column Phase
DVB
Particle Size
8 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
PL1612-1801
Name
PL1612-1801 - PLRP-S GUARD CARTRIDGES 5X3MM (X2)
LC Column Phase
DVB
Particle Size
5 um
Inner Diameter (ID)
3 mm
Length
5 mm
SKU
PL1912-1801
Name
PL1912-1801 - PLRP-S 300A 8uM 50X2.1MM
LC Column Phase
DVB
Particle Size
8 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
PL1912-1503
Name
PL1912-1503 - PLRP-S 4000A 5uM 50X2.1MM
LC Column Phase
DVB
Particle Size
5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
PL1912-1502
Name
PL1912-1502 - PLRP-S 1000A 5UM 50X2.1MM
LC Column Phase
DVB
Particle Size
5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
PL1912-1501
Name
PL1912-1501 - PLRP-S 300A 5uM 50X2.1MM
LC Column Phase
DVB
Particle Size
5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
PL1912-1500
Name
PL1912-1500 - PLRP-S 100A 5uM 50X2.1MM
LC Column Phase
DVB
Particle Size
5 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
PL1912-5801
Name
PL1912-5801 - PLRP-S 300A 8uM 250X2.1MM
LC Column Phase
DVB
Particle Size
8 um
Inner Diameter (ID)
2.1 mm
Length
250 mm
SKU
PL1912-1301
Name
PL1912-1301 - PLRP-S 300A 3uM 50X2.1MM
LC Column Phase
DVB
Particle Size
3 um
Inner Diameter (ID)
2.1 mm
Length
50 mm
SKU
PL1512-3301
Name
PL1512-3301 - PLRP-S 300A 3uM 150X4.6MM
LC Column Phase
DVB
Particle Size
3 um
Inner Diameter (ID)
4.6 mm
Length
150 mm
Key Definitions â–¸
PLRP-S
A fully polymeric reversed-phase column based on cross-linked styrene-divinylbenzene (PS-DVB) particles. Designed for extreme pH and temperature conditions where silica-based columns may fail.
PS-DVB (Styrene-Divinylbenzene)
A hydrophobic polymer matrix formed from styrene and divinylbenzene. This chemistry provides excellent mechanical strength, wide pH tolerance (1–13), and resistance to hydrolysis because it contains no silica backbone.
Polymeric Reversed Phase
A reversed-phase separation mode using a non-silica stationary phase. Polymeric RP columns provide enhanced durability under alkaline conditions and high temperatures compared to traditional C18 silica columns.
Wide pH Stability (pH 1–13)
The ability to operate in highly acidic or strongly basic mobile phases without silica dissolution or bonded-phase hydrolysis. This makes PLRP-S ideal for challenging peptide, protein, and oligonucleotide methods.
High-Temperature Operation
PLRP-S columns tolerate elevated temperatures (method dependent), improving mass transfer and reducing solvent viscosity. High-temperature capability is beneficial for large biomolecules and polymers.
Silica-Free Stationary Phase
A column packing material that does not contain silica particles. This eliminates risks of silica dissolution at high pH and expands usable method development space.
Frequently Asked Questions â–¸
What is Agilent PLRP-S used for?
PLRP-S columns are used for reversed-phase separations under extreme conditions, including high-pH, high-temperature, and aggressive solvent systems. They are commonly applied to peptides, proteins, polymers, and oligonucleotides.
How is PLRP-S different from a traditional C18 silica column?
Unlike silica-based C18 columns, PLRP-S is built on a fully polymeric PS-DVB matrix. This provides broader pH stability (pH 1–13) and eliminates silica dissolution issues at high pH. Selectivity may differ slightly due to the polymeric surface chemistry.
When should I choose a polymeric column over silica?
Choose a polymeric column like PLRP-S when your method requires strongly basic mobile phases, elevated temperatures, or long-term durability under harsh conditions. Polymeric phases expand method development space beyond typical silica limitations.
Is PLRP-S compatible with LC/MS?
Yes. PLRP-S columns are compatible with LC/MS workflows, particularly for peptides, intact proteins, and oligonucleotides where high-pH or alternative selectivity conditions may be required.
What applications benefit most from PLRP-S?
PLRP-S is widely used for protein and peptide analysis, polymer separations, oligonucleotide purification, and any application requiring broad pH tolerance and high-temperature stability.