Poroshell 300
Agilent Poroshell 300 HPLC Columns
Agilent Poroshell 300 columns are engineered for fast, high-efficiency separations of intact proteins, large peptide fragments, and biomolecules. Their unique design features a 5 µm solid-core particle with a 0.25 µm, 300 Å porous shell, delivering exceptional mass transfer and sharper peaks for high-molecular-weight analytes—all while maintaining lower backpressure than comparable wide-pore fully porous particles.
The Poroshell 300 family includes multiple bonded-phase chemistries to support a wide range of biochromatography needs:
- SB-C18 — StableBond C18 for high-temperature, low-pH operation and robust peptide/protein separations.
- SB-C8 — Reduced hydrophobicity for improved recovery of large or highly hydrophobic biomolecules.
- SB-C3 — Ideal for very large proteins and mAbs that require less hydrophobic retention for optimal elution.
- Extend-C18 — High-pH stable C18 bonded phase for enhanced flexibility and method development at elevated pH.
Together, these chemistries offer powerful selectivity options for biopharmaceutical research, proteomics, aggregate analysis, and characterization of complex protein mixtures.
For additional guidance on selecting the best Poroshell 300 phase for your workflow, contact Chrom Tech for expert recommendations.
Poroshell Particle Technology
Agilent’s superficially porous design featuring a solid core surrounded by a thin, porous shell. This reduces diffusion distance, enabling fast, high-resolution separations—ideal for intact proteins and large biomolecules in the Poroshell 300 platform.
300 Å Pore Size
Large-pore design optimized for intact proteins, mAbs, large peptide fragments, and high-mass biomolecules that require wider pore channels for efficient mass transfer.
StableBond (SB) Chemistry
Agilent’s ultra-stable, low-pH resistant bonding technology designed for extreme durability. SB phases (SB-C18, SB-C8, SB-C3) resist hydrolysis, maintain retention stability, and are ideal for acidic mobile phases.
SB-C18, SB-C8, and SB-C3 Phases
A family of ultra-stable phases optimized for proteins and peptides: SB-C18 for general protein separations, SB-C8 for hydrophobic peptides, SB-C3 for very large biomolecules requiring minimal hydrophobic interaction.
Extend-C18 Phase
A high-pH stable C18 phase featuring bidentate bonding and double endcapping, enabling separations up to pH 11.5. Ideal for peptide mapping, LC/MS workflows, and methods requiring strong retention of acidic or hydrophobic analytes under elevated pH.
What are Agilent Poroshell 300 columns designed for?
Poroshell 300 columns are engineered for fast, high-resolution separations of intact proteins, monoclonal antibodies, and large peptide fragments. Their 300 Å shell structure minimizes diffusion distance—ideal for large biomolecules.
Which Poroshell 300 chemistries are available?
The Poroshell 300 portfolio includes SB-C18, SB-C8, SB-C3, and Extend-C18—providing a full range of selectivity for intact proteins, peptides, and elevated-pH workflows.
How does the Poroshell 300 particle design improve performance?
Each particle features a solid core with a 0.25 µm porous shell, reducing analyte diffusion pathways. This results in sharper peaks, faster separations, and improved resolution for large biomolecules compared to fully porous particles.
When should I choose Extend-C18 instead of StableBond chemistries?
Choose Extend-C18 when your workflow requires high-pH separations (up to pH 11.5) or improved durability under alkaline conditions. StableBond phases are ideal for low-pH, high-temperature conditions.
What applications benefit most from Poroshell 300 columns?
These columns are ideal for intact protein profiling, mAb characterization, peptide mapping, and biotherapeutic quality testing where large molecules require fast, high-efficiency separations with minimal diffusion.
Product List
| SKU | Name | LC Column Phase | Particle Size | Inner Diameter (ID) | Length |
|---|---|---|---|---|---|
SKU
660750-909
|
Name
660750-909 - Poroshell 300SB-C3 2.1x75mm 5u
|
LC Column Phase
300SB-C3
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
75 mm
|
SKU
820999-901
|
Name
820999-901 - High performance ZORBAX guard fittings kit
|
LC Column Phase
|
Particle Size
|
Inner Diameter (ID)
4.6 mm
|
Length
12.5 mm
|
SKU
821075-924
|
Name
821075-924 - Poroshell Guard Cartridge 300SB-C3 4/pk
|
LC Column Phase
300SB-C3
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
13 mm
|
SKU
821075-920
|
Name
821075-920 - Poroshell Guard Cartridge 300SB-C18 4/PK
|
LC Column Phase
300SB-C18
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
13 mm
|
SKU
821075-918
|
Name
821075-918 - Poroshell Guard Cartridge 300SB-C8 4/pk
|
LC Column Phase
300SB-C8
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
13 mm
|
SKU
671750-902
|
Name
671750-902 - Poroshell Extend, 1x75mm, 5 micron
|
LC Column Phase
Extend-C18
|
Particle Size
5 um
|
Inner Diameter (ID)
1 mm
|
Length
75 mm
|
SKU
670750-902
|
Name
670750-902 - Poroshell Extend, 2.1x75mm, 5 micron
|
LC Column Phase
Extend-C18
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
75 mm
|
SKU
661750-909
|
Name
661750-909 - Poroshell 300SB-C3 1x75mm 5um
|
LC Column Phase
300SB-C3
|
Particle Size
5 um
|
Inner Diameter (ID)
1 mm
|
Length
75 mm
|
SKU
661750-906
|
Name
661750-906 - Poroshell 300SB-C8 1x75mm 5u
|
LC Column Phase
300SB-C8
|
Particle Size
5 um
|
Inner Diameter (ID)
1 mm
|
Length
75 mm
|
SKU
661750-902
|
Name
661750-902 - Poroshell 300SB-C18 1x75mm 5u
|
LC Column Phase
300SB-C18
|
Particle Size
5 um
|
Inner Diameter (ID)
1 mm
|
Length
75 mm
|
SKU
660750-906
|
Name
660750-906 - Poroshell 300SB-C8 2.1x75mm 5u
|
LC Column Phase
300SB-C8
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
75 mm
|
SKU
660750-902
|
Name
660750-902 - Poroshell 300SB-C18, 2.1x75 mm, 5 micron
|
LC Column Phase
300SB-C18
|
Particle Size
5 um
|
Inner Diameter (ID)
2.1 mm
|
Length
75 mm
|