Post Column Derivatization

When performing HPLC, some substances are difficult to detect by traditional UV/VIS light absorption or florescence detectors. Often times these difficult-to-detect components get lost in the background noise. Post-column derivatization (or post-column reaction) works after the separation to chemically react with the substances to give them an easily detectable physical property. For example, a strong color or fluorescent product that can easily be detected against a complex background.

Chrom Tech offers Pickering Laboratories, Inc. PCR instruments and reagents for many various applications including amino acid, mycotoxin, contaminants and residue, food and supplements, and others. For more information, reach out to our support team to determine which products will work best for your application.