Presto FF-C18

Shop Imtakt Presto FF-C18 HPLC Columns

  • High-resolution 2 µm non-porous ODS particles
  • Reversed-phase separations for bio- and synthetic polymers up to 30 MDa
  • Unique selectivity compared to traditional porous ODS columns
  • High efficiency at low flow rates, compatible with standard HPLC systems

Imtakt’s Presto FF-C18 introduces a breakthrough approach to polymer separation with an advanced 2 µm, non-porous ODS stationary phase. Traditional porous C18 columns often struggle with polymeric analytes due to inconsistent pore structures and limitations in mass transfer, leading to reduced efficiency, distorted peak shapes, and poor analyte recovery.

  • Poor peak shapes caused by broad pore size distributions
  • Loss of high-molecular-weight analytes due to entrapment in micro- and mesopores
  • Lower overall efficiency due to significant mass-transfer resistance

The Presto FF-C18 overcomes these limitations by eliminating pores entirely—allowing fast, uniform mass transfer and highly reproducible retention for polymers up to 30 MDa. Its non-porous design delivers cleaner peaks, higher recovery, and improved resolution compared to conventional reversed-phase columns. This innovative platform unlocks new capabilities in polymer analysis, biomaterials research, and next-generation separation science, offering unmatched performance for demanding modern HPLC workflows.

Key Definitions ▸
Non-Porous ODS
A stationary phase using solid, non-porous octadecylsilane (C18) particles that eliminate mass-transfer limitations associated with porous media, enabling sharper peaks and improved recovery for large polymers.
Mass Transfer Resistance
A major contributor to peak broadening in porous columns, caused by slow diffusion of analytes into and out of pores. Non-porous particles minimize this effect, enhancing efficiency and speed.
Polymer Separation
The chromatographic analysis of large biomolecules or synthetic polymers. Non-porous C18 improves resolution by preventing analyte trapping inside micro- or mesopores.
Selectivity Differences
The distinct chromatographic behavior of non-porous ODS compared to porous media, producing unique retention and separation patterns beneficial for high–molecular-weight analytes.
High-Molecular-Weight Polymers
Molecules up to tens of megadaltons (MDa). The Presto FF-C18’s poreless surface allows efficient separation of polymers up to 30 MDa without loss or peak distortion.
Frequently Asked Questions ▸

Why use a non-porous ODS column for polymer separations?

Non-porous particles eliminate analyte diffusion into micro- and mesopores, preventing peak distortion and improving recovery for high–molecular-weight polymers. This results in sharper peaks and more reliable quantitative performance.

Is the Presto FF-C18 compatible with standard HPLC systems?

Yes. Despite its advanced performance, the Presto FF-C18 operates efficiently at low flow rates, making it fully compatible with conventional HPLC instruments (no UHPLC system required).

What kinds of analytes benefit most from the Presto FF-C18 column?

Bio- and synthetic polymers, biomaterials, peptides, conjugates, and other high–molecular-weight species up to 30 MDa show dramatic improvements in peak shape, resolution, and recovery on the non-porous FF-C18 platform.