ProSEC 300S

Agilent ProSEC 300S Size Exclusion Columns

Agilent ProSEC 300S columns are engineered as a single-column solution for globular protein characterization, eliminating the need to select from multiple pore sizes or column formats. Designed specifically for high-resolution SEC analysis, these columns provide exceptional separation of monomers, dimers, trimers, and higher-order aggregates with the consistency required for modern biopharmaceutical workflows.

  • One-column solution for most globular proteins—no pore-size selection required.
  • Mechanically robust silica platform minimizes column bleed and supports long operational life.
  • Extended linear resolving range ensures accurate molecular weight determination across a broad size distribution.
  • Multiple internal diameter (ID) options allow optimization for UV, RI, and multi-detector SEC workflows.
  • Enhanced sensitivity with light-scattering detectors for confident detection of aggregates and low-abundance species.

ProSEC 300S columns are built on ultra-pure, highly stable silica optimized for biomolecule separations, allowing biotherapeutic developers to streamline method development and achieve reproducible, transferable SEC methods. Whether monitoring aggregation in monoclonal antibodies, profiling protein purity, or analyzing recombinant proteins, ProSEC 300S delivers the resolution and reliability needed for QC, R&D, and regulated environments.

For assistance selecting the best ProSEC 300S configuration for your biomolecule workflow, contact Chrom Tech.

Agilent ProSEC 300S — Phase Description ▸
ProSEC 300S
Size Exclusion Chromatography • Polymer-Based Packing • 300 Å • High Recovery for Proteins
▸

Agilent ProSEC 300S is a high-performance, polymer-based size exclusion chromatography (SEC) column designed for accurate molecular weight characterization of proteins and other water-soluble polymers. Unlike silica-based SEC columns, ProSEC 300S uses a robust polymer packing that provides excellent stability across a broad pH range and improved resistance to degradation.

When to Choose ProSEC 300S
  • You require accurate protein molecular weight determination
  • You need improved chemical stability across varied buffer conditions
  • You analyze aggregates, fragments, or oligomers
  • You want consistent performance for QC or research environments
How It Compares

Compared to silica-based SEC columns, ProSEC 300S offers enhanced pH stability and durability. It is particularly well-suited for applications requiring extended column life or operation under moderately alkaline conditions.

Key Specifications
Separation Mode Size Exclusion Chromatography (SEC)
Particle Type Polymer-based packing
Pore Size 300 Å
Application Range Proteins, water-soluble polymers, aggregates, fragments
Mobile Phase Aqueous buffer systems
Primary Use Molecular weight characterization and aggregate analysis

Practical note: Maintain appropriate ionic strength in the mobile phase to minimize secondary ionic interactions and ensure true size-based separation.

Product List

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SKU Name LC Column Phase Particle Size Inner Diameter (ID) Length
SKU
PL1547-5501
Name
PL1547-5501 - ProSEC 300S 250 x 4.6 mm
LC Column Phase
SEC
Particle Size
5 um
Inner Diameter (ID)
4.6 mm
Length
250 mm
SKU
PL1547-1501
Name
PL1547-1501 - ProSEC 300S Guard 50 x 4.6 mm
LC Column Phase
SEC
Particle Size
5 um
Inner Diameter (ID)
4.6 mm
Length
50 mm
SKU
PL1147-6501
Name
PL1147-6501 - ProSEC 300S, 300x7.5mm
LC Column Phase
SEC
Particle Size
5 um
Inner Diameter (ID)
7.5 mm
Length
300 mm
SKU
PL1147-1501
Name
PL1147-1501 - ProSEC 300S Guard, 50x7.5mm
LC Column Phase
SEC
Particle Size
5 um
Inner Diameter (ID)
7.5 mm
Length
50 mm
Key Definitions ▸
Size Exclusion Chromatography (SEC)
A chromatographic technique that separates molecules strictly by hydrodynamic size. Larger molecules elute first because they are excluded from the internal pore structure, while smaller molecules penetrate the pores and elute later.
Polymer-Based Packing
A stationary phase constructed from cross-linked polymer beads rather than silica. Polymer packings provide enhanced chemical and pH stability and improved durability under varied aqueous buffer conditions.
300 Å Pore Size
A large pore diameter optimized for the separation of proteins and high-molecular-weight polymers. This pore size allows efficient diffusion of biomolecules while maintaining accurate size-based resolution.
Hydrodynamic Radius
The effective size a molecule presents in solution, influenced by its molecular weight and three-dimensional structure. SEC separates analytes according to hydrodynamic radius rather than chemical interaction.
Protein Aggregates
Higher-order assemblies formed when protein molecules associate. SEC is commonly used to quantify aggregates and fragments during biopharmaceutical development and quality control.
Frequently Asked Questions ▸
What is Agilent ProSEC 300S primarily used for?
ProSEC 300S is designed for size exclusion chromatography (SEC) of proteins and water-soluble polymers. It is commonly used for molecular weight determination, aggregate analysis, and fragment characterization in research and QC laboratories.
How does ProSEC 300S differ from silica-based SEC columns?
ProSEC 300S uses a polymer-based packing, which provides enhanced chemical and pH stability compared to silica-based materials. This improves column durability and allows operation across a broader range of aqueous buffer conditions.
What types of samples are suitable for ProSEC 300S?
The column is optimized for proteins, biopolymers, aggregates, oligomers, and other water-soluble macromolecules. It is widely used in biopharmaceutical development and polymer analysis.
What mobile phase considerations are important for accurate SEC results?
Proper buffer selection and ionic strength are critical to suppress unintended ionic interactions. Maintaining stable pH and salt concentration ensures that separation is governed strictly by size rather than secondary interactions.
Can ProSEC 300S be used for regulatory or QC workflows?
Yes. ProSEC 300S is suitable for quality control, stability studies, and aggregate monitoring in regulated environments, offering reproducible sizing performance and robust column lifetime.