ProSEC 300S

Agilent ProSEC 300S Size Exclusion Columns

Agilent ProSEC 300S columns are engineered as a single-column solution for globular protein characterization, eliminating the need to select from multiple pore sizes or column formats. Designed specifically for high-resolution SEC analysis, these columns provide exceptional separation of monomers, dimers, trimers, and higher-order aggregates with the consistency required for modern biopharmaceutical workflows.

  • One-column solution for most globular proteins—no pore-size selection required.
  • Mechanically robust silica platform minimizes column bleed and supports long operational life.
  • Extended linear resolving range ensures accurate molecular weight determination across a broad size distribution.
  • Multiple internal diameter (ID) options allow optimization for UV, RI, and multi-detector SEC workflows.
  • Enhanced sensitivity with light-scattering detectors for confident detection of aggregates and low-abundance species.

ProSEC 300S columns are built on ultra-pure, highly stable silica optimized for biomolecule separations, allowing biotherapeutic developers to streamline method development and achieve reproducible, transferable SEC methods. Whether monitoring aggregation in monoclonal antibodies, profiling protein purity, or analyzing recombinant proteins, ProSEC 300S delivers the resolution and reliability needed for QC, R&D, and regulated environments.

For assistance selecting the best ProSEC 300S configuration for your biomolecule workflow, contact Chrom Tech.

Key Definitions ▸

Size Exclusion Chromatography (SEC)

A separation technique where molecules are resolved by hydrodynamic size. Large species elute first, while smaller molecules penetrate pores and elute later—making SEC ideal for protein, mAb, and aggregate analysis.

Globular Proteins

Compact, spherical proteins whose elution behavior is well suited to SEC. ProSEC 300S columns are designed as a single-column solution for resolving globular proteins of various sizes.

Linear Resolving Range

The molecular weight window where SEC provides accurate, proportional separation. ProSEC 300S’s extended range allows analysis of most globular proteins without needing multiple pore size selections.

Light-Scattering Detection

A detector technology used in SEC to identify aggregates, dimers, and higher-order structures with high sensitivity. ProSEC 300S columns deliver increased signal quality for multi-detector SEC workflows.

Mechanically Robust Silica

High-strength silica particles engineered to resist fracturing, reducing column bleed and supporting stable, repeatable separations—critical for reliable protein characterization.

Frequently Asked Questions ▸

What makes ProSEC 300S a single-column solution for globular protein analysis?

ProSEC 300S columns offer a wide linear resolving range optimized for typical globular protein sizes, eliminating the need to match pore sizes across multiple columns and enabling simplified SEC workflows.

Are ProSEC 300S columns compatible with multi-detector SEC systems?

Yes. ProSEC 300S columns are available in multiple internal diameters suitable for UV, RI, and light-scattering detectors, helping improve sensitivity when analyzing aggregates, dimers, and trimers.

How durable are ProSEC 300S silica particles during routine operation?

ProSEC 300S columns use mechanically robust, low-bleed silica particles that maintain stability across repeated injections, ensuring consistent performance in routine SEC workflows.

Product List

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SKU Name LC Column Phase Particle Size Inner Diameter (ID) Length
SKU
PL1547-5501
Name
PL1547-5501 - ProSEC 300S 250 x 4.6 mm
LC Column Phase
SEC
Particle Size
5 um
Inner Diameter (ID)
4.6 mm
Length
250 mm
SKU
PL1547-1501
Name
PL1547-1501 - ProSEC 300S Guard 50 x 4.6 mm
LC Column Phase
SEC
Particle Size
5 um
Inner Diameter (ID)
4.6 mm
Length
50 mm
SKU
PL1147-6501
Name
PL1147-6501 - ProSEC 300S, 300x7.5mm
LC Column Phase
SEC
Particle Size
5 um
Inner Diameter (ID)
7.5 mm
Length
300 mm
SKU
PL1147-1501
Name
PL1147-1501 - ProSEC 300S Guard, 50x7.5mm
LC Column Phase
SEC
Particle Size
5 um
Inner Diameter (ID)
7.5 mm
Length
50 mm