Protein Crash Filter Plates

Protein Crash Filter Plates for Semi-Automated Workflows

Chrom Tech’s Protein Crash Filter Plates provide a streamlined, high-throughput solution for protein precipitation and sample cleanup in bioanalytical laboratories. Designed to integrate precipitation and filtration into a single device, these plates reduce handling steps while delivering consistent, reproducible results for LC-MS and HPLC analysis.

A proprietary graded frit design efficiently removes precipitated proteins without clogging or breakthrough, producing clear filtrates ready for downstream analysis. Protein precipitation occurs directly inside each well, eliminating centrifugation and manual supernatant transfer—improving throughput while minimizing sample loss.

Key Benefits

  • Supports semi-automated protein crash workflows for increased efficiency
  • Accelerates analysis through parallel, high-throughput sample preparation
  • Improves reproducibility by cleanly removing protein precipitate via filtration
  • Eliminates manual supernatant transfer steps
  • Fully compatible with standard liquid handling systems and automated laboratory processors

These plates conform to SBS microplate standards, allowing seamless integration into existing robotic platforms and positive-pressure processing systems.

Protein Crash Filter Plate Protocol

  1. Prepare an acetonitrile-to-serum ratio of 3:1 (v/v).
  2. Spike serum with the desired analytical standard.
  3. Add the acetonitrile/serum mixture to each well.
  4. Cover the plate with a cap mat and vortex thoroughly.
  5. Plates may be vortexed with collection trays in place to prevent plate tips from contacting the vortexer platform.
  6. Extract samples using positive pressure with Chrom Tech’s 96 Positive Pressure Processor (288-0001) .
PART NO DESCRIPTION QTY
96 WELL PLATE ACCESSORIES
96-6001 Round 1 mL Collection Plate 50/cs
96-6009 Square 2 mL Collection Plate 50/cs
ZAF-PE-50 Adhesive Free Zone Sealing Film 50/cs
96-0662 Capmat for Square Well Filter Plate 50/cs
96M-03 96 Round Sealing Silicone Mat, 7 mm 10/pk
BST-9790 2 mil Blue PTFE 96-Well Pattern Film 100/cs
96 WELL PLATE INSTRUMENTATION
288-0001 Positive Pressure Manifold for 96 Well Plate ea
Key Definitions ▶
Protein Crash
A sample preparation technique that uses organic solvent (commonly acetonitrile) to precipitate proteins, allowing small-molecule analytes to remain in solution for downstream analysis.
Protein Crash Filter Plate
A 96-well filtration device designed to combine protein precipitation and filtration into a single workflow, producing clean filtrates suitable for LC-MS or HPLC analysis.
Graded Frit
A multilayer porous support structure that promotes uniform flow while efficiently trapping precipitated proteins, reducing clogging and improving sample clarity.
Positive Pressure Processing
A filtration method that applies controlled air pressure to drive samples through the plate membrane, eliminating the need for centrifugation and supporting automated workflows.
SBS Footprint
Standardized microplate dimensions that ensure compatibility with robotic liquid handlers, positive pressure manifolds, and common laboratory automation platforms.
Frequently Asked Questions ▶
What are Protein Crash Filter Plates used for?
Protein Crash Filter Plates are used for rapid protein precipitation and sample cleanup in LC-MS and HPLC workflows, enabling high-throughput processing with improved reproducibility.
Do I need a centrifuge to use these plates?
No. Filtration is performed using positive pressure, which removes precipitated proteins without centrifugation or manual supernatant transfer.
Are Protein Crash Filter Plates compatible with automation?
Yes. Plates conform to SBS standards and are compatible with robotic liquid handlers and positive-pressure processors for semi-automated and automated laboratory workflows.
What solvent is typically used for protein crash?
Acetonitrile is most commonly used, often at a 3:1 ratio with serum or sample matrix, to efficiently precipitate proteins while keeping small-molecule analytes in solution.
What makes these plates different from standard filter plates?
Protein Crash Filter Plates incorporate a graded frit design optimized for trapping precipitated proteins while maintaining consistent flow, enabling precipitation and filtration in a single device.