Reversed-Phase

Hamilton Reversed-Phase HPLC Columns

Hamilton’s reversed-phase HPLC columns are engineered to deliver exceptional inertness, longevity, and batch-to-batch consistency across a wide range of analytical workflows. By merging the strengths of both silica-based and polymeric stationary phases, these columns provide robust performance for laboratories seeking reliable separations of small molecules, peptides, pharmaceuticals, and complex organic mixtures.

Hamilton offers an extensive portfolio that includes four polymeric and two silica-based reversed-phase packing materials, each optimized for specific selectivity, retention, and chemical stability requirements. The polymeric phases provide outstanding resistance to high pH, aggressive mobile phases, and repeated cleaning cycles—ideal for high-throughput laboratories and challenging sample matrices. The silica-based phases deliver high efficiency and sharp peak shapes for traditional reversed-phase workflows, ensuring analytical precision even with demanding separations.

Together, these reversed-phase chemistries allow analysts to fine-tune methods for maximum resolution, reproducibility, and sensitivity. Whether you need rugged polymeric durability or classic silica-based selectivity, Hamilton’s HPLC columns offer reliable performance suitable for pharmaceutical, environmental, clinical, food and beverage, and research applications.

Key Definitions ▸
Reversed-Phase Chromatography
A chromatographic mode where the stationary phase is nonpolar and the mobile phase is relatively polar. Commonly used for separating organic molecules, pharmaceuticals, peptides, and other hydrophobic analytes.
Silica-Based Columns
Traditional reversed-phase columns using silica particles chemically bonded with hydrophobic ligands. They provide high efficiency and sharp peak shapes but have limited pH stability compared to polymeric phases.
Polymeric Reversed-Phase Packing
Stationary phases made from cross-linked polymeric substrates that offer superior chemical stability, extended pH range, and resistance to aggressive cleaning solvents—ideal for demanding or repetitive workflows.
Inertness
A measure of how chemically non-reactive a column’s stationary phase is. High inertness ensures minimal analyte adsorption, improved peak shape, and accurate quantitation for sensitive or reactive compounds.
Stationary Phase Selectivity
The specific ways a column’s bonded phase interacts with analytes—determining retention, resolution, and separation performance. Different Hamilton packing materials provide tailored selectivity profiles for various reversed-phase applications.
Frequently Asked Questions ▸

What advantages do Hamilton reversed-phase columns offer over traditional silica-based columns?

Hamilton combines the ruggedness of polymeric materials with the high efficiency of silica, delivering columns that are inert, long-lasting, and suitable for a wide range of reversed-phase applications. This hybrid approach improves durability, peak shape, and reproducibility.

What types of samples are Hamilton reversed-phase columns designed for?

These columns support a broad spectrum of applications including pharmaceuticals, peptides, food and beverage components, environmental extracts, and general organic compounds—making them highly versatile in analytical laboratories.

How do I choose between Hamilton’s polymeric and silica-based reversed-phase options?

Select silica-based materials when you need very high efficiency and classical reversed-phase selectivity. Choose polymeric phases for high-pH methods, aggressive solvents, longer column lifetime, or challenging sample matrices requiring enhanced chemical stability.