Scherzo C18 Mutli-Mode Column

Imtakt Scherzo C18 Multi-Mode Column

The Imtakt Scherzo C18 family (SS-C18, SM-C18, SW-C18) is a uniquely engineered multi-mode platform that integrates reversed-phase, anion-exchange, cation-exchange, and normal-phase retention mechanisms into a single column. By combining ODS (C18) ligands with both positively and negatively charged functional groups, Scherzo delivers powerful multi-dimensional selectivity not achievable with traditional single-mode phases.

This hybrid design allows analysts to resolve structurally diverse compounds—neutral, acidic, basic, and zwitterionic—in one method, making Scherzo an exceptional tool for complex mixtures, impurity analysis, metabolomics, and pharmaceutical workflows requiring broad retention control and highly tuned selectivity.

Key Definitions ▸
Multi-Mode Chromatography
A separation approach where multiple retention mechanisms—such as reversed-phase, anion exchange, cation exchange, and normal phase—can operate simultaneously or in combination, enabling exceptional selectivity for complex samples.
ODS (C18) Ligands
Octadecylsilane groups bonded to the silica surface that provide hydrophobic interactions typical of reversed-phase chromatography. Scherzo combines these with ionic ligands for enhanced selectivity.
Anion-Exchange Mode
A chromatographic mode in which positively charged ligands attract and retain negatively charged analytes. Scherzo incorporates anion-exchange sites to extend retention for acidic compounds.
Cation-Exchange Mode
A mode where negatively charged ligands retain positively charged analytes. Scherzo’s dual-ligand chemistry allows simultaneous reversed-phase and ion-exchange selectivity.
Normal Phase Mode
A chromatographic mechanism based on polar interactions, typically retaining polar analytes more strongly. Scherzo supports normal-phase-like retention due to its mixed-mode functional groups.
Frequently Asked Questions ▸

What makes the Scherzo C18 a multi-mode column?

Scherzo integrates ODS (C18) ligands with both anion- and cation-exchange groups, enabling reversed-phase, anion-exchange, cation-exchange, and even normal-phase-like retention in a single column—something not possible with conventional stationary phases.

What types of compounds benefit from the Scherzo’s mixed-mode selectivity?

Scherzo excels at separating mixtures containing acids, bases, neutrals, and zwitterions. It is particularly effective for complex pharmaceutical mixtures, metabolomics samples, impurities, and analytes that are difficult to resolve using single-mode C18 phases.

How does Scherzo differ from a conventional C18 column?

Conventional C18 columns rely solely on hydrophobic interactions. Scherzo adds dual ionic functional groups, creating a multi-dimensional selectivity profile that enables finer retention control and the resolution of diverse analytes in one method.